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    2025-03-21

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      ???? ?????????, ??? ??? ????, ????? ??? ???? ???? ??. ??? ???? ??? ?? ????? ???. ???? ?????? ??? ????? ????? ????? ?????????? ??? ??? ??? ?? ?????? ???? ??? ??? ??? ???? ????? ??, ?? ????? ??? ??? ???? ??? ??? ????, ????? ????? ?????? ?? ????? ?????? ??? ???? ??? ??? ??. ????? ???? ??? ??? ??? ???? ??? ?????? ?????? ????? ???? ??? ????? ??? ???? ????? ???? ???? ??? ????? ??? ???? ?????? ?????? ??? ??? ?????, ??, ?????, ??? ??? ??? ??? ????.??? ??? ??? ???? ???? ??? ???? ?? ?? ??? ?????, ??? ??, ???? ???? ?? ????. ????? ???? ???, ???? ???-?? ???? ????? ??????? ? glycoproteins ???? ??? ?????. ??? ??? ????? ????? ???? ???????? ???? ???? ??? ??????? ???? ?? ????? ????, ???? ????? ??? ?? ???? ???? ??? ?? ??? ??? ???? ????? ?? ????? ??????. ????? ???? ????? ??, ???? ?? ???? (????) ?????, ? sorbitol isomers ??, ??? ??? ????, ?? ????? ???, ????? ?? ????? ??? ??? ??? ???. ????? ??? ??? ??? ??? ?? ???? ?????? ?????? ????? ??? ??? lyophilized ???? ????? ???? ????. ????? ????? ?? ??? ?????? ??? ????? ?? ???-??? ???? ?? ????? ??, ?? ???? ????? ?? ?????? ????? ?? ?????. ?????? ????? ????? ???? ?????? ???? ????? ???? ???????? ????? ?????? ????? ???. 1% ??? ??? ??? ??? ????? ???? ????? ??????? ????? ??????? ???? ?????? ???? ?? ? ??? ?? ???? ??? ??? ????? ????????? ?? ????? ?????? ????? ??????? ??????? ????? ? 4 ??? ??? ??? ??? ???? ???? ?? ?? ?? (1 ???? ???) ????? ???? ???? ???? ?? ???? ????? (??? 0.1% BSA? 5%HSA? ??? 10% FBS ?? ???? ?????? ??? ?????) ?? ? -20℃ ??? -80℃ ?? ????? ??? ???? ????? ?????? ????? ????? ?? ???? ????? ????? ??, ?????? ?????? ???? ?? ???? ????? ??? ??? ??????. ????? ?? ????? ???? ?? ? 8% (???? ????) ??? lyophilized ??????? ????? ????? ????? ???? ??? ????? ???? ?? ? lyophilization ??? ????? ???? ???? ?? ????? ????? ????? ????? ????????? ??? ?????? ?????? ???? ???? ???? ???? ??? ?? ??? lyophilized ????? ?? ??? ??? ???? ?????? ????? ?? ????? ???? ????? ???? ? ?????-????? ?? ??? ????? ??? ?????? ????? ? ?? ????? ???? ??????? Porosity ????? ?? sublimation ??? ??? ??, ?? ???? ????? ????? sublimate ????, ???? ??? ?????? ?? ???? ????. ???? ????? ???? ??? ?? ?????? ??? ????? (???? ???? ??? Tp?Tg' ??? Tc) ??? ????? ?? ?????????? lyophilization ???? ????? ??? ???? ?????? ????? ??????? ???? ????????? ????? ???? ?? ??????? ?? ???? ??? ???? 1?2 ??? ????? ?????? ??? ??????? ??? ???? ???? ????? ??? ????. ??? ???? ?????? ???? ?????? ????? ??? ???? ???? ????? ?? ????? ?? ?? ????? ???? ?? ?????? ?????? ??? ???? ????? ? Tg' (??? ? Tc) ?? ?? ???? ??? ????? ????? ????? ????. ????? ???