Ua hō?ike ?ia nā ha?awina mua he nui nā pōmaika?i olakino e like me ka ?ōnaehana pale, nā hopena antioxidant, a me ke olakino cardiovascular. ?Oiai he mea?ai ko?iko?i ka hua?ai C no ka mālama ?ana i ke olakino, hiki i ka nui a i ?ole ka li?ili?i ke loa?a nā hopena maika?i ?ole i ke olakino.

?O ka Melanoma (MM) he ma?i ma?i ?ino e puka mai ana i loko o nā ?ili pua?a o ka ?ili a ?o ia ke ?ano pō?ino loa o ka ma?i ma?i ?ili, ?oiai he ha?aha?a kona ma?i a ?oi aku ka huhū a me ka metastatic. Ke pi?i nei ka ma?i o ka melanoma i nā makahiki i hala iho nei.

I kēia mau lā, ua ho?opuka nā mea noi?i mai ke Kulanui o South Florida ma ?Amelika Hui Pū ?Ia a me ke Kulanui o Leicester ma United Kingdom i kahi pepa i kapa ?ia ?o "Redox modulation of oxidatively-induced DNA damage by ascorbate enhances" i loko o ka puke pai Free Radical Biology and Medicine ma ka vitro a me ex-vivo DNA ho?okumu ?ia a me ka make cell i nā melanoma cell ".

Ua hō?ike ?ia nā ha?awina ?o ka mālama ?ana i nā ma?i kanesa melanoma me ka huaora C hiki ke ho?onui i ka pō?ino DNA i ho?oiho ?ia e ka oxidant i nā pūnaewele ma?i a ho?oikaika i ka make ?ana o ka ma?i ma?i, a ua kūlike kēia pō?ino i ka nui o ka melanin i loko o nā pūnaewele. A no nā ?ili ?ili ma?amau, pā?ani ia i kahi hana pale.

I loko o kēia noi?i, ua ho?okumu nā mea noi?i i kahi pū?ulu o nā laina cell MM me ka pigmentation ?oko?a, ho?ohana i ka hydrogen peroxide ma ke ?ano he oxidant kumu ho?ohālike, a ho?opa?a ?ia ka huaora C e ho?onui i ka make ?ana o ka melanoma cell ma ka ho?onui ?ana i ka pō?ino DNA i ho?oiho ?ia.

Ua ho?ā?o ka po?e noi?i i nā pae o ka pō?ino DNA i ho?okomo ?ia i ka vitamina C i loko o ?elima mau laina kelepona a ?ike ?ia ua ho?ohālikelike ?ia me nā keratinocytes (HaCaT), ke ki?eki?e o ka pō?ino DNA endogenous i nā cell MM āpau, i ke ?ano o ka pō?ino nui: SK23 cell me ka pigmentation ki?eki?e, SK28 cell with moderate pigmentation, A375P a me A375M cell me ka loa?a ?ole o ka pigmentation DNA.

Eia kekahi, ua nānā nā mea noi?i i ka na?au o nā laina kelepona ?elima i ka pō?ino i ho?okomo ?ia e kahi oxidant (hydrogen peroxide) a ?ike ?ia ua kūlike ka na?au o ka hydrogen peroxide me ka mea i luna.

Ua hō?ike hou ?ia ka nānā ?ana o nā ?ano oxidative intracellular e hō?ike ana nā cell MM i nā ki?eki?e endogenous ki?eki?e o nā ?ano oxidative intracellular ma mua o nā cell HaCaT, a ua kūlike ke ka?ina o nā laina kelepona ?elima me ka pō?ino DNA, ka na?au pō?ino, a me ka cytopigmentation.

Ma hope a?e, ua mālama nā mea noi?i i nā cell me ka ?ole o ka huaora C a nānā i nā hopena ho?oponopono kūpono o ka hua?a C ma ka ho?omohala ?ana i ka hō?ino DNA a me ka pepehi ?ana i nā cell.

Ua hō?ike ?ia nā hopena no nā cell MM āpau, ua ho?onui nui ?ia ke ki?eki?e o ka pō?ino DNA endogenous i ho?oulu ?ia e ka mālama ?ana i ka huaora C, ?oiai ?a?ole nui ka nui o nā cell HaCaT, a ua kūlike ka pō?ino DNA endogenous me ka mea i luna.

Eia kekahi, ?oi aku ka ki?eki?e o ka nui o ka pō?ino nucleobase i ho?okomo ?ia i ka huaora C i loko o nā pūnaewele SK23 ki?eki?e (18.5%) a ha?aha?a loa i nā pūnaewele A375P non-pigmented (14.2%).

Ma muli o ka hiki i ka huaora C ke ho?onui i ka pō?ino o ka DNA endogenous a me ka pō?ino nucleobase i loko o nā cell MM, ua loiloi nā mea noi?i i ka hopena o ka huaora C ma ka DNA double strand breaks a ?ike ?ia no nā cell MM āpau, pi?i nui ke ki?eki?e o ka DNA double strand breaks induced by vitamin C treatment, akā ?a?ole no nā cell HaCaT. ?O ke ka?ina o nā laina kelepona ?elima ke kūlike me ka mea i luna.

?O ka mea nui, ua ?imi ka po?e noi?i inā ho?onui ka huaora C i ka make ?ana o ka MM cell peroxid-induced, a ?ike ?ia ua ho?onui ka huaora C i ka pepehi ?ana i ka peroxid-induced i nā cell MM āpau, ?oiai ke pā?ani nei i kahi kuleana pale i nā cell HaCaT, a ua kūlike ke ka?ina pepehi me ka mea i luna.

?O ka hope, ua ?ike pū ka ha?awina e hiki i ka huaora C ke ho?onui i ka pono o ka lā?au melanoma e loa?a nei ?o Elesclomol, e ho?onui nui i ka pō?ino DNA o nā pūnaeka ma?i ma?i i ho?oulu ?ia e Elesclomol.

Ua ?ōlelo ka po?e noi?i, ?o ka ho?ohana ?ana i ka huaora C, hiki ke ho?onui i ka pō?ino DNA i loko o nā pūnaewele ma?i ma?i a alaka?i i ka make ?ana o ka ma?i ma?i ma?i, he ala ?oi aku ka maika?i o ka mālama ?ana i ka melanoma, kahi e pono ai nā noi?i a me nā ho?okolohua hou e hō?oia ai.

Ma muli o ka a?o maika?i ?ia o ka huaora C a ua ?ike ?ia e ?ae maika?i ?ia, mana?o ka po?e noi?i hiki i nā kauka ke ho?ohana i ka vitamina C i mea ho?ohui e ho?omaika?i i nā lā?au lapa?au.

I hui pū ?ia, hō?ike kēia ha?awina in vitro i kēlāhua?ai Chiki ke ho?oikaika i ka oxidation-induced endogenous DNA poino, paipai i ka ma?i ma?i 'a?ai make, oiai e pā?ani ana i ka hana pale i loko o ma?amau ili kino, a ho?onui i ka pono o ka melanoma lā?au lapa?au, e pono e a?o hou.