Any? niile maara na nr?gide na-ad?te aka nwere ike ?kpata ?t?t? nsogbu ah?ike an? ah? na nke uche, d? ka nchekas?, ?da mbà n'obi na mmet?ta nd? ?z? na-ad?gh? mma, yana nsogbu ag??, nsogbu ibu ar? na nsogbu nd? ?z? nke metabolic. ?t?t? nd? mmad? na-eche ihu nr?gide, ga-egosi iri nri mmet?ta uche (nri mmet?ta uche), ya b?, site na iri oke iji belata mmet?ta uche na-ad?gh? mma nke nr?gide kpatara. A na-esonyekar? iri nri mmet?ta uche na oke calorie, nke b? otu n'ime ihe nd? na-akpata oke ibu. Yab?, kedu usoro nke nr?gide na-ebute nsogbu ag??? Kedu njik? d? n'etiti metabolism tryptophan na nri mmet?ta uche kpatara nr?gide? Iji dozie nsogbu nd? a, nd? na-eme nch?p?ta emewo usoro nyocha zuru ezu.

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Tryptophan b? otu n'ime amino acid d? mkpa d? na ah? mmad? na onye na-ebute neurotransmitter 5-hydroxytryptophan (5-HT, makwaara d? ka serotonin) na ?b?r?. ?n? ?g?g? d? ukwuu nke ?m?m? egosila na nsogbu metabolic tryptophan nwere njik? chiri anya na ?r?a d? iche iche nke uche, d? ka ?da mbà n'obi, nchekas?, schizophrenia na nd? ?z?. N'otu oge ah?, tryptophan na metabolite 5-HT na-etinyekwa aka na nhazi nke ag?? na nguzozi ike. Ya mere, n'okpuru ?n?d? nr?gide na-ad?gh? ala ala, tryptophan metabolism na-agbanwe? Mgbanwe a ?? met?tara omume iri ihe na-akpata nr?gide na-ad?gh? mma? N'iburu aj?j? nd? a n'uche, nd? nch?p?ta ah? mere usoro nyocha zuru ezu.

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Nke mb?, nd? nyocha ah? jiri ?m? oke mep?ta ?d? nri nri mmet?ta uche na-akpata nr?gide na-ad?gh? ala ala (CMS). Ha dobere ?m? oke nd? ah? n'?d? nr?gide d? nro na-enwegh? at?, d? ka nr?gide mgbochi, ?l? ?ma jijiji, na ebe ?sa mmiri oyi, maka ?b?ch? 21 n'usoro iji mee ka nr?gide na-ad?gide ad?gide, d? iche iche na nd? n'ezie. Nsonaaz? gosiri na mgbe ?b?ch? 21 nke nr?gide na-ad?gh? ala ala, ?m? oke ah? gosip?tara oke nchegbu na omume ?da mbà n'obi. N'ime nnwale oghere (OFT), oge ?m? oke nd? otu CMS na-an? na etiti mpaghara mepere emepe belatara nke ukwuu, na-egosi na ?kwa nchegbu ha abawanyela. N'ime nnwale nlegide ?d? (TST), oge mgba ?m? oke na otu CMS belatara nke ukwuu, na-at? aro mm?ba nke enwegh? olileanya. N'otu oge ah?, nri ?m? oke na CMS m?bara nke ukwuu, mana ibu ah? belatara nke ukwuu, na-at? aro na ag?? na metabolism ha na-akpaghas?.

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Mgbe nke ah? gas?r?, nd? nch?p?ta ah? mere nyocha metabolomic ezubere iche maka ?z? metabolic tryptophan na serum òké. Nsonaaz? gosiri na ?d?naya tryptophan serum nke ?m? oke CMS belatara nke ukwuu, ebe metabolites 5-hydroxytryptamine (5-HT) na ?d?naya kynurenine na-abawanye nke ukwuu. Nnyocha nd? ?z? gosiri na okwu mRNA nke tph1, b? isi enzyme na njik? nke 5-hydroxytryptamine, b? nke a na-ach?kwa nke ?ma n'ime an? ah? nke ?m? oke na otu CMS, na ?d?naya nke 5-HT na colon gosikwara ?n?d? ala. Nke a na-egosi na nr?gide na-ad?gh? ala ala na-akpaghas? homeostasis nke ?z? usoro metabolic tryptophane-5-HT na eriri af? ?m? oke, na-ebute enwegh? ahagh? nhata nke ?kwa 5-HT d? n'ak?k?.

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? b? ezie na tryptophan enwegh? ike ?gafe ihe mgbochi ?b?r? nke ?bara ozugbo, metabolite 5-HT ya na-ar? ?r? d? mkpa d? ka neurotransmitter na sistem? akwara ma na-etinye aka n'?hazi ?t?t? usoro physiological d? ka ?n?d?, cognition na iri nri. Nd? nch?p?ta ah? nyochara mgbanwe na nkwup?ta nke ?t?t? neuropeptides na nd? na-anabata 5-HT na-etinye aka na nch?kwa ag?? na hypothalamus. Nsonaaz? gosiri na nr?gide na-ad?gh? ala ala kwalitere okwu nke ag?? na-akwalite neuropeptides d? ka AgRP na OX1R, ebe ? na-agbadata okwu nke ihe na-egbochi ag?? d? ka LEPR, MC4R na 5-HT1B. Nke a na-egosi na ?gba aghara nke ?z? tryptophane-5-HT nwere ike ibute omume nri na-ad?gh? mma site na-emet?ta sekit akwara hypothalamic.

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Yab?, mgbakwunye na tryptophan ? nwere ike belata nr?gide na-ad?gh? ala ala nke kpatara ?n?d? na omume iri nri? A na-enye ?m? oke CMS doses nke tryptophan ab?? (100mg/kg na 300mg/kg) site na ntanye kwa ?b?ch? maka ?b?ch? 21. Nsonaaz? gosiri na mgbe ?b?ch? 21 nke ntinye aka na nnukwu dose of tryptophan (300mg/kg), nchekas? na ?da mbà n'obi-d? ka omume ?m? oke na nnwale n'?h?a na nnwale nkwusioru ?d? ka emelitere nke ?ma, na mm?ba na-ad?gh? mma na oriri na ?nw? na-edozi. Nnyocha nd? ?z? egosiwo na ntinye aka nke tryptophan d? elu nwere ike igbochi nhazi elu nke ihe nd? na-akwalite ag?? d? ka AgRP na OX1R na hypothalamus kpatara nr?gide na-ad?gh? ala ala, ebe ? na-eweghachi okwu nke ag?? na-egbochi ihe nd? d? ka LEPR, MC4R, 5-HT1B na 5-HT2C. ? d? mma ?mara na n'agbanyegh? na obere dose nke tryptophan (100mg/kg) egosighi mm?ba d? ukwuu na ngosip?ta omume, enwere mgbanwe mgbanwe na mkp?r? nd? ihe met?tara ag?? hypothalamic na ?kwa molekul.

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Ihe omumu ihe omumu ihe omuma nke ozo gosiputara na 5-HT, site na ijikota 5-HT1B na 5-HT2C anabata na hypothalamic POMC, AgRP na neurons ndi ozo, na-egbochi neurons AgRP / NPY nke na-akwalite ag??, na-eme ka POMC neurons nke na-egbochi onu ogugu ma na-akwalite oriri ike, ma na-egwu egwu. Nke a na-enye nkwado usoro mkp?r? nd? d? mkpa maka mmelite nke nri mmet?ta uche site na ?z? tryptophan-5-HT.

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Nd? ?r? nyocha ah? jikwa eriri hypothalamic neuron cell GT1-7 iji ch?p?ta n'ihu nyocha usoro iwu nke 5-HT na neuropeptides met?tara ag??. Ha na-emeso mkp?r? nd? GT1-7 na 10μM nke corticosterone (CORT) maka awa 24 iji mee ka ?n?d? nr?gide na-ad?gh? ala ala. Nsonaaz? gosiri na ngosip?ta nke ag?? na-akwalite mkp?r? nd? ihe nketa d? ka AgRP na OX1R b? nke a na-ach?kwa nke ?ma site na ?gw?gw? CORT, ebe okwu ag?? na-egbochi mkp?r? nd? ihe nketa d?ka MC4R, 5-HT1B na 5-HT2C na-ach?kwa. Mgbe emechara ya na 0.1μM 5-HT maka awa 2, enwere ike gbanwee okwu na-ad?gh? mma nke CORt-induced AgRP na mkp?r? nd? ihe nketa nd? ?z?, na enwere ike iweghachi okwu nke MC4R, 5-HT1B na 5-HT2C. Nke a kwuputara mmet?ta nch?kwa kp?mkwem nke 5-HT na neurons hypothalamic.

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Nch?k?ta data:

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Mmet?ta nke nr?gide na-ad?gh? ala ala na tryptophan metabolism homeostasis na ?m? oke: nr?gide na-ad?gh? ala ala mere ka ?kwa tryptophan serum belata (P

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Mmet?ta nke tryptophan na ag?? na ?da mbà n'obi-d? ka omume nke nr?gide na-ad?gh? ala ala kpatara: Mgbakwunye tryptophan d? elu weghachiri omume iri nri na-ad?gh? mma na oke ibu nke nr?gide na-ad?gh? ala ala (P

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Mmet?ta nke tryptophan supplementation na hypothalamic feeding neurons na ag?? na-ach?kwa ?m? oke nr?gide na-ad?gh? ala ala: Immunohistochemical staining gosiri na okwu nke c-fos na AgRP na mpaghara ARC nke hypothalamus na-ad?gh? ala ala na-abawanye ?ba, ebe okwu LEPR na-ebelata nke ukwuu (P

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Mmet?ta nke tryptophan na ?z? metabolic 5-HT na hypothalamus nke ?m? oke nr?gide na-ad?gh? ala ala: Serum tryptophan na 5-HT na-abawanye nke ?ma mgbe mgbakwunye tryptophan (P

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N'ozuzu, ?m?m? ihe a na-ekpughe na nr?gide na-ad?gh? ala ala na-akpata nkw?s? nke netw?k nch?kwa ag?? hypothalamic site na-akpaghas? ?z? metabolic tryptophane-5-HT, nke na-ebutekwa iri nri mmet?ta uche. Mgbakwunye nke tryptophan exogenous, kar?s?a na nnukwu doses (300mg / kg), na-eweghachi ?kwa 5-HT nke etiti, na-eme ka hypothalamic 5-HT1B na 5-HT2C nd? na-anabata ya, na-egbochi agRP / NPY neurons, na-eme ka POMC neurons r?? ?r?, ma na-eme ka nchekas? na-eri nri na omume ?j??.

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Nsonaaz? nyocha a nwere uru bara uru d? mkpa. N'ime nd? ?gbara ?h?r? na nd? nr?gide d? elu nke oge a, ?t?t? nd? na-eche nsogbu mmet?ta uche na-akpata nr?gide na nsogbu ibu ibu ihu. Nnyocha e mere egosiwo na nr?gide ogologo oge na-eme ka ?kwa tryptophan d? n'ah? na-agbada, njik? 5-hydroxytryptamine na-ebelata, wee na-eduga n'?t?t? nsogbu nke neuroendocrine, na-eduga na ?da mbà n'obi, nchekas? na mmet?ta nd? ?z? na-ad?gh? mma, yana oke ag??, oke ibu na nsogbu nd? ?z? metabolic. ?m?m? ihe a na-ekpughe ?r? d? n'etiti tryptophan na metabolite 5-hydroxytryptophan n'ime axis-mmet?ta-ag?? ag??, na-enye echiche na ?z? ?h?r? maka iwep? nchekas? na imeziwanye nsogbu ?n?d? uche na nsogbu ibu.

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Dabere na nchoputa ah?, nd? nch?p?ta na-enye ?f?d? nd?m?d? nri iji nyere nd? mmad? aka ?nagide nr?gide nke ?ma. Nke mb?, nri kwes?r? ekwes? nke tryptophan bara ?ba na nri kwa ?b?ch?, d? ka àkwá, cheese, mkp?r?, unere, oatmeal, wdg, nwere ike inye aka melite ?kwa nke tryptophan n'ime ah?, kwalite njik? nke 5-hydroxytryptophan, si otú ah? meziwanye ?n?d? mmet?ta uche na igbochi iri nri. Agbanyegh?, ekwes?r? iburu n'uche na tryptophan na nri anagh? etinye 100% ma tinye ya n'?r?. N'otu oge ah?, ogologo oge nnukwu dose supplementation nke tryptophan (d? ka ihe kar?r? 500mg/kg) nwere ike ?kpata mmebi ak?r?. Ya mere, na nguzozi nri kwa ?b?ch?, mgbakwunye obere nke tryptophan nwere ike ?b?, akwadola iji ihe mgbakwunye tryptophan gabigara ókè.

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Na mgbakwunye, mmega ah? na-agafegh? oke d? ka ?gba mwe mwe ?s?, igwu mmiri, yoga, wdg, nwekwara ike inye aka ?bawanye ?kwa nke tryptophan na njik? serotonin n'ime ah?, belata nchekas? ma melite ?n?d?. ?ra ?ra nke ?ma, ?z?lite àgwà ziri ezi na inwe nchekwube, na ?m?ta ikwup?ta mmet?ta uche n'?z? ezi uche d? na ya b? ?z? nile d? irè isi nagide nr?gide. Mgbe ? na-eche nsogbu mmet?ta uche siri ike ihu d? ka ?da mbà n'obi na nchekas?, ? d? mkpa ?ch? ?gw?gw? ?kachamara ?kachamara n'oge.

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N'ikpeaz?, ?m?m? a na-ekpughe usoro nke iri nri mmet?ta uche nke nr?gide na-ad?gh? ala ala na-akpata site n'echiche nke tryptophan metabolism, ma na-enye echiche ?h?r? maka ibelata nchekas?, na-eme ka nsogbu ?n?d? uche na nsogbu d? ar?. ? b? ezie na a ch?r? ?m?m? ihe ?m?m? nd? ?z? iji kwalite usoro onunu na ogologo oge nke mgbakwunye tryptophan na nri, nsonaaz? nke ?m?m? a enyela any? echiche ?h?r? maka ?kwalite ah?ike an? ah? na nke uche site na nhazi nri. Any? kwenyere na site na nri kwes?r? ekwes?, mmega ah? na-agafegh? oke, ezigbo ?ra na mmegide mmet?ta uche, any? nwere ike iji nway?? na-eche nr?gide nke nd? ihu ma nabata ?d?nihu ka mma.