????? ??? ?? ????, ???? ????, ???? ??? ??????. ???? ?????? ???? ??? ?? ????. ??? ???? ????? ???? ????????? ??????, ?????? ?????? ???????????? ?? ??????? ????????. ?????? ??? ?????? ???? ????, ???? ????? ?? ??? ??? ???? ??? ??? ???? ??????, ????? ??????? ????? ?????? ??????, ??????? ??????? ????? ??? ?????? ????. ????? ???? ???? ????? ?????? ???? ????? ???, ???? ????? ???? ???? ???? ???????? ?????? ?????? ?????. ????, ???? ???? ???? ????? ???? ???????, ??? ???????, ??????, ?????? ????. ???? ????, ???? ???? ??? ?????? ????, ???? ????, ??????? ???????. ?????? ???, ???? ????? ??????? ?? ?????????????? ???????? ?? ????? ?????? ????????????. ?????? ??????? ?????? ???? ?? ???? ???? ???? ?????? ????? ????? ????, ???? ??? ?????? ?????? ???? ?? ???? ?????? ?????? ?????? ?????? ?? ?????? ????? ????. ?????? ??? ??????? ????, ????? ???? ??? ????? (?????), ??? ?????? ?? ????????, ???? ????, ???? ?????? ????, ??? ??? ???? ????? ???????. ?????? ???? ???? ?? ?? ????? ??? ?????, ??? ?? ???? ????? lyophilized ??????? ???????. ?????? ????? ?? ?????? ?? ????? ????? ??????? ?????? ?????????? ???, ?????? ?????? ???? ??????????? ?? ??????. ???? ?????? ???????? ?????? ?? ?? ???????? ?? ????? ????? ???? ?????? ????? ???? ?? ?????? ????. ?????? ?????? ?? 1% ?? ???? ?????? ??????? ?? ?????? ????? ??????? ????? ????? ????????, ?? ??????? ?????? ?? ?????? ????? ?????? ????? ?? ??????? ?????? ?????. ???? ??? ????????, ?????? ?-4°C ??? ?? ????? ??? (???? ????) ???? ?????? ????. ?????? ????? ????, ????? ????? ?????? (??????? ????? ?????? ???, ???? 0.1% BSA, 5% HSA ?? 10% FBS) ????? ??? ??-????? ????? ?-20℃ ?? -80℃. ???? ?????? ?????? ?????? ?????, ??? ?? ????? ?????? ????? ????? ????? ???? ?? ??????. ?????? ??????? ???????? ???? ??? ?? 8% (??? ??? ???) ?????? ??????. Trehalose ???? ????? ????? ?? ?????? ????? ????? ?? ????? ??? ?????? ????????? ?? ????? ????? ????????????. ?????? ??? ?? ???? ??? ????? ????? ???? ????? ???? ????? ?????? ?? ??????? ?? ??????? ??????? ???? ???????????. ???? ?????? ???? ???? ?? ????? ?????? ????? ??????? ??????, ????? ????? ?????? ?? ??? ????? ??????? ?????? ?????? ?? ???? ?????????. ???????? ??? ???? ????? ?????????? ?? ??? ??????, ????????? ?????? ???? ?????? ????? ????????? ??? ????, ?? ????? ?????? ?? ??? ?????? ???????. ?????? ?? ????? ????? ?????? ??? ????? ?????? ??? ???? (???????? ????? Tp?Tg' ?? Tc) ????? ?????? ?? ???? ????? ?? ????? ????????????? ????????????. ???? ??? ???? ??? ????? ?????? ???? ????? ????? 1:2 ??? ???? ?? ???????? ?????? ?? ???? ??????. ????? ?????? ???? ????? ????? ??????? ????? ???????. ??? ?-Tg', ???? ??????? ????? ?? ??? ????? ????? ???? ???? ????? ??? ????? ????? ??? ?????? ?? ??? ??? ???????. ????? ?????? ?????, ???? ????? ????? ????? ????????? ????? ?????? ?-Tg' (?? ????? Tc). ???????? ?? ?????? ????? ???? ?-50 ?"?/?"?, ????? ????? ????? ????? ?????? ?????? ??-Tg ?? ?????? ????? ???????? ?? ?????? ????? ?????? ????. ?? ???, ?? ???? ?????? ???? ?????? ????? ????? ?????????????, ?????? ?????? ????? ????? ?????? ??? ??????? ?????????? ????? ??????, ?? ????????? ?? ???? ?????? ?????? ???? ??? ?? ??? ????? ??? ?????.