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    D-mannose: Kuchokera ku mphatso yokoma ya chilengedwe kupita ku ulendo wabwino kwambiri wopanga

    2025-03-13

    2. Shuga ndi mutu wodziwika komanso wovuta m'miyoyo yathu yatsiku ndi tsiku. Kuchokera pa sucrose wamba, fructose kupita ku D-mannose yodziwika bwino, lero, tikambirana za nyenyezi yotsika kwambiri koma yotheka kwambiri - D-mannose.

      D-mannose ikhoza kumveka ngati dzina losadziwika bwino, koma kwenikweni ndi hexose yachilengedwe yomwe imapezeka kwambiri m'chilengedwe. Mosiyana ndi sucrose ndi fructose, D-mannose ikubwera pamaso pa anthu ndi kukoma kwake kwapadera komanso ubwino wambiri wathanzi. Kutsekemera kwake kuli pafupifupi 60% ya sucrose, ndi shuga wochepa wa calorie wogwira ntchito, chifukwa chofuna kudya zakudya zabwino kwa anthu amakono, mosakayika ndi chisankho chabwino.

      D-mannose sikuti amangokoma, komanso mtengo wake ndi wodabwitsa. M'munda wazakudya, zitha kugwiritsidwa ntchito ngati chokometsera chochepa cha calorie popanga zakudya zosiyanasiyana zathanzi; Pa nthawi yomweyi, chifukwa cha antioxidant, anti-inflammatory and other properties, monga moisturizer ndi anti-aging zosakaniza zimagwiritsidwa ntchito kwambiri mu zodzoladzola. Pazachipatala, zawonetsa kuthekera kodabwitsa, ndipo kafukufuku wasonyeza kuti D-mannose imatha kuchepetsa kutupa kwamatumbo, kuchiza matenda a nyamakazi, kupewa asthmatic airway kutupa, komanso kuwonetsa zotsatira zabwino pochiza khansa ndi matenda obadwa nawo a glycosylation.

      M'zaka zaposachedwa, ndi chitukuko chofulumira cha biotechnology, njira yachilengedwe yakhala njira yodziwika bwino yopangira D-mannose. Njirayi imagwiritsa ntchito michere yachilengedwe kuti ipangitse zomwe zimachitika kuti zisinthe mashuga ena (monga D-fructose) kukhala D-mannose, yomwe ili ndi zabwino zambiri, kuteteza chilengedwe komanso mtengo wotsika.

      Pakati pa ma enzyme ambiri achilengedwe, D-mannose isomerase imakondedwa chifukwa cha mphamvu yake yayikulu yothandizira. Komabe, maphunziro ambiri oyambilira adagwiritsa ntchito Escherichia coli ngati ma cell a chassis popanga, zomwe zimathandizira kupanga bwino komanso kubweretsa zoopsa zachitetezo. Kupatula apo, E. coli si vuto lazakudya, ndipo mankhwala omwe amapangidwa amakhala pachiwopsezo cha kuipitsidwa kwa endotoxin. Pofuna kuthetsa vutoli, asayansi anayamba kufufuza mitundu ina yotetezeka. Pamapeto pake, adasankha Bacillus subtilis ngati cell ya chassis yatsopano. Bacillus subtilis adakopa chidwi kwambiri chifukwa cha mawonekedwe ake osagwiritsa ntchito poizoni, osavulaza, oletsa mabakiteriya osavuta, osavuta kukhalapo komanso osavuta kupanga kukana mankhwala. Chofunika koposa, chadziwika bwino kwambiri ngati mtundu wa "General Recognized as Safe" (GRAS) womwe umakwaniritsa zofunikira paukhondo wazakudya.
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      Mu phunziro ili, poyerekeza ndi enzymatic katundu wa D-mannose isomerase ku magwero angapo, gwero mulingo woyenera kwambiri mannose isomerase potsiriza anasankhidwa ndi heterologous anasonyeza mu Bacillus subtilis, ndi recombinant mavuto B. subtilis 168 / pMA5-EcMIaseA anamangidwa bwinobwino. D-mannose idapangidwa ndi cell catalysis yonse pogwiritsa ntchito D-fructose ngati gawo lapansi. Kutembenuka ndi zokolola za D-mannose zidasinthidwanso ndikuwongolera kutentha kwa kutembenuka, pH ndi ndende ya gawo lapansi. Mulingo wa 5 L fermenter umathandizira kaphatikizidwe kabwino ka D-mannose ndi kutembenuka kopitilira 27% ndi zokolola zopitilira 160 g/L. Kupambana kumeneku sikunangophwanya mbiri yakale yopanga, komanso kunapereka chithandizo champhamvu chaukadaulo pakupanga mafakitale a D-mannose.
      E-mannose, mphatso yokoma iyi kuchokera ku chilengedwe, ikukopa chidwi cha mafakitale osiyanasiyana ndi chithumwa chake chapadera komanso mphamvu zopanda malire. Ndi chidwi chochulukirachulukira chazakudya chathanzi komanso biotechnology, chiyembekezo chamsika wa D-mannose chikukula kwambiri. M'tsogolomu, tili ndi chifukwa chokhulupirira kuti D-mannose idzagwira ntchito yofunika kwambiri m'madera ambiri ndikukhala mphamvu yofunikira kulimbikitsa chitukuko cha makampani azaumoyo. Nthawi yomweyo, tikuyembekezeranso kuti asayansi apitilize kuphunzira mozama za biosynthesis ndi kagwiritsidwe ntchito ka D-mannose, ndikutibweretsera zodabwitsa komanso zopambana.