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  1. ???? ?? ???????? ?????? ?? ?????? ??? ???? ????? ?????? ????????, ?????????? ???? ????? ??? ???? ??????? ??-?????? ?????????, ??? ??? ?? ??? ?????? ??????? ???????? ???? - ??-?????? ?????? ?????? ??????

    ??-?????? ?? ??????? ????? ??? ??? ????????, ?????? ??? ???????? ?? ????????? ???????? ???? ????????? ???? ????? ???????? ???????? ??? ?????????? ??? ?????, ??-?????? ???? ????? ???? ??? ?????? ????????? ??? ???? ?????? ????? ?????? ???? ???? ???????? ? ????? 60%, ??? ?? ???-???????? ??????????? ????, ?????? ?????? ???? ????? ????? ????, ?????????? ?? ?? ??????

    ??-?????? ???? ????????? ?????, ??? ???? ?????? ????? ???? ?????????? ????? ?????????, ????? ??????? ????? ????? ??????? ???? ?? ???-???????? ???? ????? ??????? ???????????; ???? ?????, ???? ???????????????, ??????-?????? ??? ???????? ??? ?????, ?? ?????????? ????? ??? ????????? ??????????-?????? ?????? ?????? ????? ??????? ???? ???????? ?????????, ??? ???????????? ???????? ???????, ??? ???????? ???????? ?? ??-?????? ????????? ??????? ????? ????? ????????, ????????? ???????????? ???????? ????????, ?????? ?????? ???????? ?????????, ??? ????? ??? ?????? ?????????????? ?????? ?????????? ???? ?? ????? ???????????

    ?????????? ????????????, ??? ?????????? ????? ????? ????, ????? ?????? ???? ???? ??-?????? ???????? ?????????? ?????? ???????? ??? ?????? ????? ????????????? ??????? ??? ????????????? ???????? ??? ???? ???? (??????? ??-??????????)?? ??-???????? ????? ??????, ????? ???? ??????, ?????? ??????? ??? ?? ?????? ?????? ??????

    ???? ????? ?????? ??????, D-mannose isomerase ???? ???? ?????????? ?????? ????? ????? ??????? ?????, ??????? ?????????? ???????? Escherichia coli ?? ??????? ???? ?????? ??? ????? ??????? ??????????, ???? ??????? ???????? ????? ??????? ???????? ??????? ???? ???? ???????? ???????, E. coli ?? ?????-???????? ??????? ?????, ??? ??? ??????? ??????? ???????? ?????? ???????????? ???????? ?? ???????? ???? ?????? ???? ??? ??????? ?????? ????, ????????????? ???????? ?????? ??????? ?????? ????? ???? ?????, ?????? ???? ?????? ??? ????? Bacillus subtilis ?? ??????? Bacillus subtilis ???? ??-???????, ?????????, ???? ??????????????????, ????? ??? ????? ??? ??? ???????? ??????? ????? ??? ????? ???? ???? ????????? ????? ???? ????? ?????? ?????? ???? ?????????????? ??? ?????, ????? "???????? ?????? ????? ??????" (GRAS) ??????? ????? ?????? ????? ???????? ???????? ???? ????? ???????? ???????? ???? ????
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    ??? ????????, ?????? ?????? D-mannose isomerase ? ??????????? ?????????? ????? ???, ????? mannose isomerase ? ????????? ?????? ??? ?????????? ??? Bacillus subtilis ?? heterologous ?????? ??????????, ??? ??????????? ???????? B. subtilis 168/pMA5-EcMIaseA ?????? ?? ??????? ???????? D-mannose ?? ?????????? ????? D-fructose ??????? ??? ?????????? ??? ?????????? ?????? ????????? ??????????? ???????? ?????????, pH ??? ?????????? ??????????? ?????????? ??? D-mannose ? ???????? ??? ??????? ????? ????? ??????????? 5 L ?????????? ???? 27% ?? ???? ???????? ??? ??? 160 g/L ?? ???? ??????? ???? D-mannose ? ???? ?????????? ????? ??????? ??? ????? ???? ????? ??????? ?????? ???????????, ??? D-mannose ? ????? ??????? ???? ???? ?????? ?????? ???? ?????? ????????
    ???????? ??? ???? ?????, ?-??????, ???? ????? ?????? ??? ???? ???????? ???? ??????? ?????? ?????? ?????? ?????? ????? ????? ??? ??? ??????????????? ????? ???????? ????? ????, ??-?????? ? ???? ???????? ?????? ???? ?????? ?????? ??????? ?????????, ?? ????? ??????? ?????? ???? ??? ?? ??-?????? ???? ????????? ?? ?????????????? ?????? ????? ???? ??? ????????? ?????? ??????? ??????????? ????? ???? ?? ?????????????? ????? ???? ???? ?????, ??? ??? ???? ??? ????? ?? ????????????? ??-?????? ? ??? ???????? ??? ?????? ???????? ???? ?????? ???? ????????? ??? ???? ???? ????????? ??? ????? ??????????