??? ?????? ???? ?? ?????????? ??? ??????? ??? ?????? ????????? ??????? ?? ??????? ?????? ???????, ??????? ??????, ????????? ??? ???????? ????????? ?????, ??? ??? ?????, ??? ????? ??? ???????? ?????????? ??????? ???? ???????? ??? ???? ??? ???????? ????? (???????? ?????) ???????, ???????, ??? ?????? ?????? ??????? ????????? ??????? ??? ????? ???? ??????? ????? ?????? ???????? ????? ??????? ???? ???????? ???? ???, ???? ???????? ???? ???? ?????? ?????? ????, ??? ?????? ??? ????? ?'? ???? ???????????? ???????????? ??? ???-???????? ???????? ????? ?????? ?'? ??????? ???? ??? ????????????? ?????? ????, ????????? ??????? ?????????? ?? ??????? ?????????

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???????????? ????? ???? ?????? ???? ?????????? ????? ????? ?????? ????? ??? ?????????? ????????????????? 5-??????????????????????? (5-HT, ?????? ?????????? ???? ???????) ? ?????????? ??? ?????? ???????? ???????? ?? ???????????? ???????????? ?????? ??????? ?????? ??? ???? ?????? ????? ????, ??????? ?????????, ??????, ????????????? ???????? ???? ?????, ???????????? ??? ???? ??????????? 5-HT ???? ??? ??? ????? ??????? ??????????? ?????? ????, ?????????? ??? ???????????, ???????????? ???????????? ?????????? ??? ??? ??? ?????????? ?'? ?????????? ???-??????????? ????? ???? ???? ????? ??? ????????????? ???? ???, ????????? ??????? ???????? ?? ??????? ???????? ????????

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??????, ????????? ?? ?????????? ???-??????? ???????? ????? ????? (CMS) ?????? ????? ???? ???? ??????? ???????? ?????? ??????????? ??????? ????? 21 ??? ???? ??????? ??????? ??????? ??????????? ???, ??????? ????? ???, ??????? ?????? ??? ????? ???? ?????, ?????? ?????? ???????, ????? ????? ?????? ????? ???? ???????? ??????????? ???????? ?? 21 ???? ?????????? ??? ???, ???????? ?????????????? ?????? ??? ????????? ??? ???? ???????? ???????? ???? ??????? ??????? (OFT) ??, ???? ???????? ????????? ??????? CMS ??????? ???? ?????? ??? ?????? ????? ??????????, ???? ????????? ?? ????????? ?????? ???? ?????? ?????? ????? ??????? ??????? (TST) ??, CMS ??????? ??????????? ?????? ??? ?????? ????? ??????????, ???? ??????? ???????? ??????? ?????????? ???? ?????, CMS ??????? ??????????? ????? ????? ?????? ?????? ?????, ?????? ??? ?????? ????? ?????, ???? ????????? ?? ????????? ??? ??? ???????????? ?????? ????????

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????????? ?????, ????????? ???? ?????? ???????????? ?????????? ?????? ?? ?????????? ???????? ???????? ??????? ???????? ?? CMS ?????? ????? ????? ???????????? ?????? ?????? ????? ???????, ???????? ???????????? ???????????? 5-???????????????????????? (5-HT) ??? ???????????? ?????? ?????? ?????? ???????? ???? ?????????? ???????? ?? 5-???????????????????????? ?????????? ?? ?????? ??????? tph1 ? mRNA ??????? CMS ??????? ????? ????? ?????? ?????? ????? ??????? ??? ???????? 5-HT ? ?????? ???? ????????? ????????? ?????????? ??? ????? ??? ?? ?????????? ??? ????? ??????? ???? ????????????-5-HT ?????????? ?????? ????????????? ?? ???? ???, ???? ???? ????? 5-HT ????? ???????? ?????? ????

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???? ???????????? ??????? ????-???????? ???? ??????? ??????? ?????, ???? ??????????? 5-HT ????????? ?????? ????????? ?? ?????????????????? ????? ?? ?????????????? ?????? ????? ??? ??? ??????, ????? ??? ????? ??? ???? ??????? ??????????? ????????? ??????? ????? ????????? ???????????? ?? ??? ??????????? ???? ???? ??????????????? ??? 5-HT ????????? ????????? ???????????? ????? ???????? ????????? ??????????? ???????? ?? ?????????? ??? AgRP ??? OX1R ??? ????? ??????????? ??????? ????????????????? ????????? ????????? ????? ?????? ???????, ???????? LEPR, MC4R ??? 5-HT1B ??? ??? ????????? ??????????? ????????? ????? ???????? ??? ????? ??? ?? ????????????-5-HT ?? ????????? ????????????? ???????? ???????? ???????? ??? ?????????? ????? ???? ?????? ????????

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????, ???????????? ???? ??????? ??????? ???-??????????? ?????? ??? ????? ???? ??????????? ??? ???????? ??? 21 ??? ???? ????? ?????? ?????? CMS ???????? ???????????? ? ????? ???? (100mg/kg ??? 300mg/kg) ??????????? ??????? ???????? ?? 21 ??? ??? ???????????? ? ???? ???? (300mg/kg) ???? ????????? ????? ???, ???? ??????? ??????? ??? ????? ???????? ????????? ??????????? ?????? ??? ????????? ??? ?????? ?????? ?????? ???????, ??? ?????????? ????? ?????? ??????? ????? ????? ??? ??? ????? ?????? ??????????? ???? ???????? ???????? ?? ????-???? ???????????? ????????? ??????? ??? ?????? ??????? ???????????? ?? AgRP ??? OX1R ??? ??? ?????????? ??????????? ??????-??????????? ???? ???????, ???????? LEPR, MC4R, 5-HT1B ??? 5-HT2C ??? ??? ????????? ??????????? ????????? ??????????? ???????? ??? ????????? ?? ???? ???????????? ??? ?????? (100mg/kg) ?????? ???? ???????????? ????????? ?????? ????? ?????, ????? ????? ?????? ????????????? ??? ????????? ???? ????????? ??????????? ???? ?????

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???? ????? ?????????? ???????? ???????? ?? 5-HT, 5-HT1B ??? 5-HT2C ??????????????? ????????????? POMC, AgRP ??? ???????? ??????? ???? ??????, ????? ??????????? ??????? AgRP/NPY ??????????????? ???? ???, ????? ???? ??????? ??? ????? ????????? ??????????? ??????? POMC ??????????????? ?????? ???, ??? ??’??? ??? ??? ??? ????????? ??????? ?? ?????? ????? ???? ??? ????????????-5-HT ?? ?????? ???????? ?????? ?????? ???? ?????????????? ????? ?????????? ?????? ?????? ????

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????????? ??? ????????? ???????????????? ???? 5-HT ? ?????? ???????? ????? ????? ????? ???? ????? ????????????? ??????? ??? ????? GT1-7 ???? ??????? ???????? ?????? ?????????? ??? ??????????? ?????? ????? ???? 24 ????? ???? 10μM ?????????????? (CORT) ???? GT1-7 ?????????? ???????? ???????? ??????????? ?????????? ?? AgRP ??? OX1R ??? ??? ??????????????? ?????? ??????? CORT ???????? ?????? ????????? ????? ?????? ???????, ???????? MC4R, 5-HT1B ??? 5-HT2C ??? ??? ????????? ?????? ??????? ????? ???????? 0.1μM 5-HT ???? 2 ????? ???? ?????? ???????? ???, CORt-??????? AgRP ??? ???????? ?????? ?????????? ????????? ?????? ????? ????????????, ??? MC4R, 5-HT1B ??? 5-HT2C ? ????????? ??????????? ???????????? ??? ????????????? ??????? ???? 5-HT ? ????????? ?????? ???????? ????? ??????? ????????

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??????????? ???????????? ???????????? ????????????? ???? ?????????? ???? ??????: ?????????? ??? ????? ????? ???????????? ???? ????? ????? (P

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?????????? ??? ?????? ??????? ??? ??? ???????? ??? ???? ???? ???????????? ??????: ????-???? ??????????? ?????????????? ?????????? ??? ?????? ??????? ?????????? ????? ???? ??? ??? ??????? ??????????? ??????? ??(P

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??????? ???????? ?????? ????????????? ????? ??????? ??? ??? ????????? ???? ???????????? ???????????????? ??????: ????????????????????? ??????? ??????? ?? ??????? ???????? ????????? ?????????????? ARC ??????? c-fos ??? AgRP ? ??????? ?????? ?????? ???????, ???????? LEPR ? ??????? ?????? ????? ??????? (P

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??????? ???????? ????? ??????????????? 5-HT ?????????? ????? ???? ???????????? ??????: ???????????? ?????????????? (P

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???????? ?????, ??? ???????? ???????? ?? ?????????? ??? ?????????????-5-HT ?????????? ???? ???? ??? ????????????? ??? ?????? ?????????? ???? ?????? ???, ???? ???? ???????? ????? ????? ????? ???? ?????????? ???????????? ???????, ???????? ???? ???????? (300mg/kg), ????????? 5-HT ?????? ??????????? ???, ????????????? 5-HT1B ??? 5-HT2C ??????????????? ?????? ???, AgRP/NPY ????????????? ???? ???, POMC ????????????? ?????? ???, ??? ???-????????? ?????? ????? ??? ?????????? ????? ?????? ????? ????

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??? ?????? ?????? ?????????????? ????????? ??????? ?????? ???? ????????, ??????? ?????????? ??????, ???? ??? ???-???????? ???????? ?????? ??? ??? ?????? ???????? ????????? ???????? ???????? ?? ?????????? ??? ????? ???????????? ?????? ????? ??????, 5-???????????????????????? ???????? ????? ??????, ??? ??’??? ?????????????????? ??????? ?? ??????? ?????? ??????, ???? ?????????, ?????? ??? ???????? ????????? ?????, ??? ?????????????, ????????? ??? ???????? ?????????? ?????? ?????? ??????? ??? ?????? ???-?????-?????? ????????? ?????? ???????????? ??? ???? ??????????? 5-??????????????????????? ? ????????? ?????? ?????? ??????, ??? ??? ????? ??? ?????? ????? ??? ??? ??????? ????? ????? ???? ???? ????? ??? ?????? ?????? ???????

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??? ???????? ???? ???? ???, ????????? ??? ???? ?? ????? ???????? ????? ???? ???? ????? ??????? ?????? ??????? ???????, ???????? ??????? ??????????? ????? ?????? ??????? ???????, ??????? ?????, ????, ?????, ????, ????, ???????, ?????? ???????????? ?????? ????? ??????? ??????? ???????, 5-??????????????????????? ?????????? ??????????? ???????, ?????????? ???????? ???????? ?????? ?????? ??? ???????? ????? ??????? ???? ??????? ?????, ??? ????? ???? ???? ?? ??????? ??????????? 100% ????? ??? ??????? ??? ?????? ???? ?????, ???????????? ?????????? ??? ???? ??????? (??????? 500mg/kg ?? ????) ????? ?????? ???? ???????? ????, ???????? ????? ?????????, ???????????? ????? ??????? ???????, ??????????? ???????? ??????? ????????? ?????? ?????? ??????

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??? ??????, ????, ???????, ??? ??????? ????? ??????? ???? ?????? ???????????? ??? ?????????? ????????? ???? ?????? ???????, ??? ??? ??????? ??? ?????? ????? ??????? ??????? ???????? ?????????? ??? ?????, ?? ????????? ??? ??????? ?????? ?????? ????? ??? ??????????? ????? ????? ?????? ????? ?????? ????? ??? ???? ???????? ?????? ?????????? ????? ???????? ????????? ??? ?????? ??? ?????? ???????? ??????? ???????? ???????, ???????? ??? ?????? ???????? ????????????? ???????? ???? ???????

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?????, ??? ?????? ???????????? ???????????? ??????????? ?????????? ??? ?????? ??????? ???????? ?????? ?????? ????????? ?????? ??? ??? ????? ??????, ?????? ????? ??? ??? ??????? ????? ????? ???? ?? ???? ????????? ?????? ???? ???? ??????? ???????????? ???????????????? ?????? ??? ?????? ?????????? ????? ???? ????? ???????? ?????? ??????, ??? ??????? ????? ???? ????? ????????? ???????? ??????? ??? ?????? ??????????? ??????????? ????? ???? ???? ????? ?????? ?????? ??? ??????? ??? ?? ?? ???????? ?????, ????? ???????, ?? ??? ??? ????????? ???????? ???????? ????????, ??? ????? ????? ???? ????? ????? ?????? ????????? ??? ?? ????? ????????? ????? ??????????