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    2025-03-13

    2. ???? ??? ???? ????? ??????? ?????????? ?? ??????? ?????????. ?????????, ???????? ??? ????? ????????? ???? ??-?????? ?????, ?? ??? ??? ?????? ??? ????? ??? ???? ???????? ?? ??-?????? ??? ?.

      D-mannose ??? ?????? ????? ?????? ??? ??????, ?? ???? ??????? ???????????? ????? ????? ????? ???????? ???????? ???????. ????????? ?? ???????? ???? ???, D-mannose ??? ???????? ???? ?? ?? ??? ?????? ????????? ?????? ???? ??????? ??? ??. ??? ???? ?? ????????? ????? 60% ?? ??? ?? ??? ?? ??? ????? ???? ??????????? ???? ???????, ???? ????? ???? ?????? ??????? ???? ????? ??????? ????? ????, ??????? ??? ??????? ??.

      D-mannose ?????? ???????? ????? ???, ??? ?????? ??????? ? ???? ????. ???? ??????????, ?? ????? ?????? ??????? ???? ????????? ???? ??? ????? ????????? ??? ?????? ?? ?????; ? ????, ??? ???????????????, ??????-???? ??????? ?? ??????? ?????? ????, ????????????? ???? ??? ?? ?????? ??? ?????????? ??????????? ?????????? ?????????? ????? ?????? ??. ?????? ????????? ???, ?? ????????? ??????? ???????? ?? ??? ???, ???????????? ?????? ?? ????? D-mannose ????? ???? ??????? ?????? ??? ??? ??????, ?????????? ????????? ????????? ??????, ???? ????? ??????? ??????? ?????????? ?? ?????? ?? ???????? ??????????????? ???? ???? ????????? ???????? ??? ??? ???????? ???????? ?? ???? ????.

      ??? ???????, ??? ????????? ?????? ????????? ???, ??? ??????????? ?????? ????????? D-?????? ?????????? ??????? ?????? ?????? ??? ???? ??. ??? ?????? ????? ??????? ???? (D-???????? ????) D-?????? ??? ????????? ????? ???? ?????????????? ??????????? ?????? ??? ??????????? ??????? ?????? ????, ??? ??? ????????????, ???????? ??????? ?? ??? ??????? ?? ???? ??.

      ???? ??? ??????????? ??????? ???, D-mannose isomerase ??? ??? ?????????? ??????? ???? ?????? ????. ???? ?????, ???? ?????????? ???????? ????????? ???? ???? ??? ??? Escherichia coli ?????? ?? ??? ?????? ???????? ???????????? ???? ?????? ?? ??? ??????? ??????? ? ??? ???. ??????? ???, E. coli ????-??????? ???????????? ???? ???, ?? ?????? ??????? ???????? ????????????? ????? ???? ???? ???????? ??? ????. ??? ?????? ?????? ????, ???????????? ??????? ?????? ????????? ?????? ???? ????. ???????, ????? ?? ???? ???? ??? Bacillus subtilis ???? ????. ??? ????, ?????? ????, ????????? ???????????????, ???? ?????? ???? ???? ?? ??? ?????????? ???????? ???? ???? ??? ????? ???? Bacillus subtilis ???? ??????? ???? ??. ????? ?????? ??? ???, ?? ???? ?????????? ???????? ??????? "???????????? ??????? ??? ????????" (GRAS) ???????????? ??? ?????? ??? ??????? ??.
      1 ??
      ??? ????????? ??, ??? ????????????? D-mannose isomerase ?? ??????? ?????? ???????? ????????, mannose isomerase ?? ???????? ??????? ??????? ???? ????? ?? ??? Bacillus subtilis ?? ????????? ??????????? ??? ?? ??? ???, B. subtilis 168/pMA5-EcMIaseA ???? ?????? ?????????? ??????? ?????? ??. D-fructose ????????? ??? ?????? ?????? ???????? ??? ??????????? ????? D-mannose ????????? ??? ???. ???????? ????????, pH ??? ?? ?????? ?????????? ???????? ????? ????? D-mannose ?? ????????? ?? ???????? ???????? ?????????? ??? ???. 5 L ??????? ????? 27% ? ???? ???????? ????????? ?? 160 g/L ? ?????? ?????????? ??? D-mannose ?? ????????? ????????? ??????? ????. ??? ????????? ??? ???????? ??????? ??? ?????? ????? ???, D-mannose ??????? ????????? ???? ???????? ???????? ???? ? ??? ???????.
      ?????????????? ?????? ??? ?????? ??????? ?? E-mannose, ??? ???????? ???????? ?? ?????? ????? ??? ????? ?????????? ?????? ??????? ?? ??????? ????. ?????? ??????? ???? ????? ?? ??? ???????? ?????? ?????? ???????? ???, D-mannose ?? ?????? ???????? ?? ????? ???????? ?????? ????. ?????????, D-mannose ???? ?????????? ?????? ??????????? ??? ???? ??? ???? ?????? ?????????? ???????? ?????????? ????? ???? ?????? ???????? ??? ?????? ??? ???? ??????? ?????? ??? ???? ????. ? ????, ????????????? D-mannose ?? ??? ????????? ?? ?????? ?????????? ???????? ???????? ?????? ?? ??? ???? ????? ?? ????? ????? ??? ??? ???? ??? ????? ??? ??????? ????.