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D-mannose: Kubva kuchipo chinotapira chechisikigo kuenda kurwendo runoshamisa rwekugadzira
2025-03-13
- Shuga inyaya inozivikanwa uye yakaoma muhupenyu hwedu hwezuva nezuva. Kubva kune yakajairwa sucrose, fructose kusvika kudiki-inozivikanwa D-mannose, nhasi, isu tichataura nezve yakaderera-kiyi asi inogona kuve nyeredzi - D-mannose.
D-mannose inogona kunzwika sezita risingazivikanwe remubatanidzwa, asi iro chairo hexose yemusikirwo inowanikwa zvakanyanya mune zvakasikwa. Kusiyana ne sucrose uye fructose, D-mannose iri kuuya muziso revanhu nekutapira kwayo kwakasiyana uye hutano hwakawanda hunobatsira. Kutapira kwaro kunenge 60% ye sucrose, ishuga yakaderera-calori inoshanda, nekuda kwekutsvaga kudya kune utano hwevanhu vemazuva ano, pasina mubvunzo sarudzo yakanaka.D-mannose haisi kungonaka zvinonaka, asiwo kukosha kwayo kwekushandisa kunoshamisa. Mumunda wekudya, inogona kushandiswa seyakaderera-calorie sweetener yekugadzirwa kwezvakasiyana-siyana zvekudya zvine hutano; Panguva imwecheteyo, nekuda kweiyo antioxidant, anti-inflammatory uye zvimwe zvinhu, semunyoro uye anti-kukwegura zvigadzirwa zvinoshandiswa zvakanyanya muzvizoro. Munharaunda yezvokurapa, yakaratidza kukwanisa kunoshamisa, uye zvidzidzo zvakaratidza kuti D-mannose inogona kuderedza chirwere chinoputika, kurapa rheumatoid arthritis, kudzivirira asthmatic airway kuzvimba, uye kunyange kuratidza migumisiro yakanaka mukurapa kenza uye congenital glycosylation disorders.
Mumakore achangopfuura, nekukurumidza kukura kwebiotechnology, nzira yebhayoloji yave zvishoma nezvishoma yave nzira huru yekugadzira D-mannose. Iyi nzira inoshandisa ma enzymes ehupenyu kukonzeresa maitiro ekushandura mamwe mashuga (akadai seD-fructose) kuita D-mannose, ine zvakanakira kushanda kwakanyanya, kuchengetedza kwezvakatipoteredza uye mutengo wakaderera.
Pakati peakawanda ma enzymes ehupenyu, D-mannose isomerase inofarirwa nekuda kwehukuru hwayo hwekuita. Nekudaro, mazhinji ezvidzidzo zvekutanga akashandisa Escherichia coli sechassis maseru ekugadzira, ayo akavandudza kugadzirwa kwakanaka asi zvakare akaunza njodzi yekuchengetedza. Mushure mezvose, E. coli haisi yekudya-yakachengeteka dhizaini, uye makemikari ainogadzira anounza njodzi inogona kuitika yekusvibiswa kwe endotoxin. Kuti agadzirise dambudziko iri, masayendisiti akatanga kutsvaga mamwe marudzi akachengetedzeka. Pakupedzisira, vakasarudza Bacillus subtilis sero itsva chassis. Bacillus subtilis yakwezva kutarisisa zvakanyanya nekuda kwehunhu hwayo hwekusaita chepfu, kusakuvadza, kunoshanda antibacterial, isiri nyore kuramba uye isiri nyore kugadzira kurwisa zvinodhaka. Zvinotonyanya kukosha, yave ichizivikanwa zvakanyanya se "Inowanzozivikanwa seyakachengeteka" (GRAS) dhizaini rinosangana nehutsanana hwekudya.
Muchidzidzo ichi, nekuenzanisa iyo enzymatic properties yeD-mannose isomerase kubva kune dzakawanda zvinyorwa, iyo yakakwana sosi yemannose isomerase yakazosarudzwa uye heterologous yakaratidzwa muBacillus subtilis, uye recombinant strain B. subtilis 168 / pMA5-EcMIaseA yakagadzirwa zvinobudirira. D-mannose yakagadzirwa nesero rese catalysis uchishandisa D-fructose se substrate. Iko kutendeuka uye goho reD-mannose zvakawedzera kuvandudzwa nekugadzirisa tembiricha yekutendeuka, pH uye substrate concentration. Iyo 5 L fermenter level inogonesa kuumbwa kwakanaka kweD-mannose ine shanduko inodarika 27% uye goho rinopfuura 160 g/L. Kubudirira uku hakuna kungotyora rekodhi rekutanga rekugadzira, asi zvakare yakapa rutsigiro rwakasimba rwehunyanzvi hwekugadzira indasitiri yeD-mannose.E-mannose, ichi chipo chinotapira kubva kune zvakasikwa, chiri kukwezva kutarisa kwemaindasitiri akasiyana nerunako rwayo rwakasiyana uye risingaperi kugona. Nekuwedzera kwekutarisa kune hutano hwekudya uye biotechnology, tarisiro yemusika yeD-mannose iri kuramba ichiwedzera kuvimbisa. Mune ramangwana, isu tine chikonzero chekutenda kuti D-mannose ichaita basa rakakosha mune mamwe minda uye kuva simba rakakosha kusimudzira indasitiri yehutano. Panguva imwecheteyo, isu tinotarisirawo kuti masayendisiti anogona kuramba achidzidza zvakadzama biosynthesis uye mashandisirwo ekushandisa kweD-mannose, uye kutiunzira zvimwe zvinoshamisa uye kubudirira.