0102030405
Mannose uye Mannitol
2025-03-21
- Kuomesa nechando, kunonzi kuomeswa nechando, inzira yekuomesa iyo inofanoomesa mushonga wemushonga unoda kuomeswa kuita wakasimba uye wobva wadzikisa mvura kubva munzvimbo yechando pasina kupinda munzvimbo yemvura pasi petembiricha yakaderera uye yakaderera. Nokuti iyo yose yekushanda maitiro iri mumamiriro ekupisa, nzira iyi inonyanya kukodzera kugadzirira kupisa-sensitive protein drug preparations. Mushonga weprotein wakaomeswa nechando waive wakasununguka sekeke, iro rakanga risingangobatsiri kuchengetedza chete asiwo raibatsira pakudzoreredzwa kweprotein drug mushure mekuperazve. Zviripachena, tekinoroji yekuomesa-inoomesa inopa nzira inoshanda yekugadzirira yeprotein mishonga yekugadzirira ine isina kugadzikana yemuviri uye makemikari zvinhu. Nekudaro, iyo yechando-kuomesa maitiro inonetsa yekuchinja kwechikamu, uye kune zvakawanda zvinhu zvinokonzeresa mapuroteni denaturation mukuita kutonhora, kunyungudika-kunyungudika, kuomesa uye kuchengetedza, saka mamwe majekiseni ekudzivirira anowanzoshandiswa kudzikamisa puroteni mune inonyorerwa. Shuga, iyo inonyanya kushandiswa excipient muzvigadzirwa zvakaomeswa nechando, boka rehydroxyl muchimiro charo rinogona kutsiva chisungo chehydrogen pakati peprotein nemvura kuti chipe mhedzisiro yakagadzikana, isiri-chaiyo stabilizer yeprotein, uye inogona kuita chimwe chikamu chekudzivirira mumatanho akasiyana-siyana ekuomesa nechando (sekuomesa nechando, kuomesa-kunyorova, nezvimwewo). Kudzivirira kweshuga kune hukama nerudzi rweprotein uye disaccharides, uye ma disaccharides ndiwo anonyanya kudzidzwa uye anozivikanwa anonyanya kushanda ekudzivirira. Sucrose is a disaccharide inoumbwa nemorekuru imwe yeglucose uye imwe molecule yefructose, iyo yakagadzikana kemikari uye kazhinji ine amorphous chimiro, uye inoita basa rakakosha mukudzivirira chechipiri chimiro shanduko yemapuroteni, uye kuwedzera uye kuunganidzwa kwemapuroteni panguva ye lyophilization uye kuchengetedza. Kuenzaniswa ne sucrose, trehalose ine yakakwirira girazi tembiricha tembiricha, yakaderera mwando induction, uye kushoma kudzikisira. Aya mabhenefiti anoratidza kuti trehalose inogona kunge iine yakakura application tarisiro. Doro, rakadai semannitol, rinowanzoshandiswa semafirita anopenya pakutonhora kunononoka kupa chimiro chekutsigira chechikamu chinoshanda uye usaite nazvo.
Mannose is monosaccharide, soluble mumvura, zvishoma soluble mu ethanol. Pasina ruvara kana chena crystalline poda. Inoita basa rakakosha mumetabolism yevanhu, kunyanya mu glycosylation process yemapuroteni chaiwo. Zvakaoma kuti mannose inyudzwe nemuviri wemunhu, uye inozoburitswa nemuviri wemunhu mushure mekudya, kunyanya kuburikidza neitsvo metabolism, uye iyo yese metabolic cycle ingangoita maawa masere. Kudya mannose hakukonzeri kukwira kweshuga, saka imhando yeshuga inogona kudyiwa nevamwe vane chirwere cheshuga. Muzvisikwa, mannose iripo mune yakasununguka mamiriro mune mimwe michero, yakadai secranberries, maapuro, maranjisi, etc. Mumuviri wemunhu, mannose inoparadzirwa mumatumbu ose neropa, kusanganisira ganda, nhengo uye nerve. Mune aya matishu, mannose inobatanidzwa mukuumbwa kweglycoprotein inogadzirisa basa re autoimmune system. Ongororo dzekiriniki dzakapfuura dzakaratidza kuti mannose inogona kurapa uye kudzivirira weti turakiti hutachiona, saka zvimwe zvekunze zvigadzirwa zvehutano zvine mannose sechinhu chikuru chinoshandiswa kuchengetedza weti system hutano. Mannitol idoro reshuga, rinowanzoderedzwa kubva kushuga (mannose), isomers ye sorbitol, inonyunguduka mumvura, white crystalline powder, ine inonaka inonaka yakafanana ne sucrose. Mannitol inogona kushandiswa kwete chete seyakanakisa skeleton agent, asiwo se lyophilized protein inodzivirira mune mamwe mishonga. Kudzivirira kwemannitol paprotein kune hukama nekugadzirisa kwayo uye morphological chimiro, uye kusungirirwa kwayo dzimwe nguva kune hukama nekristaro morphology. Zvinowanzotendwa kuti amorphous mannitol ine basa rekudzikamisa mapuroteni ukuwo mannitol yakavhenekerwa ichirasikirwa nebasa rayo rekudzivirira. Mannitol concentrations ye1% kana zvishoma inodzivirira kuunganidzwa kweprotein zvinodhaka kuburikidza nekuumbwa kweamorphous maumbirwo, asi yakanyanya kuwanda kwemannitol kunowanzo kupenya uye kukurudzira kuunganidzwa kwemishonga yemapuroteni. Kune akawanda mapuroteni, kuchengetedza pa4 ° C inguva pfupi chete (inenge vhiki 1) mushure mekumiswazve. Nekuchengetedza kwenguva refu, zvinokurudzirwa kugadzirira diluents (anofanira kunge aine mapuroteni anotakura, senge 0.1% BSA, 5% HSA, kana 10% FBS) uyezve subpack yakaomeswa nechando pa -20 ℃ kana -80 ℃. Zvakakosha kudzivisa kuomesa nechando kakawanda uye kunyunguduka, sezvo kutonhora kwega kwega uye kunyunguduka kunokonzera kusashanda kweprotein. Trehalose uye mannitol zvinowanzowedzerwa ne8% (mass by volume) sevanodzivirira lyophilized. Trehalose inogona kudzivirira zviripachena shanduko yechipiri chimiro cheprotein uye kutambanudza uye kuunganidzwa kweprotein panguva ye lyophilization. Mannitol zvakare inoshandiswa zvakanyanya lyophilized inodzivirira uye inozadza iyo inogona kuderedza kuunganidzwa kwemamwe mapuroteni mushure me lyophilization. Iyo yekuzadza inogona kupa chimiro chakakodzera chekeke yakaomeswa nechando, inobatsira kune chimiro chakakodzera chechando-yakaomeswa keke block, uye kugadzira iyo pore chimiro. Porosity chinhu chinodiwa pakuderedzwa kwemvura yakaomeswa nechando, uye mapores akakura anogona kunyura nekukurumidza, saka nguva yekutanga yekuomesa inogona kuderedzwa. Kuwedzerwa kwemafirita kunogonesa kupfupika kwekutanga kuomeswa (chigadzirwa tembiricha Tp?Tg' kana Tc) nguva uye inogona kushandiswa senzira yekukwirisa iyo biopharmaceutical lyophilization process. Kazhinji chiyero che stabilizer kune filler chinofanirwa kunge chiri 1: 2 kupa iyo inodiwa crystallization yekuzadza. Iyo latisi inokonzeresa inopa tsigiro yemagetsi kune izvo zvikamu zviri mune yavo amorphous state. Pamusoro peTg', iyo amorphous state yakaumbwa negadziriro yegadziriro haina kuomarara zvakakwana kuti itsigire huremu hwayo uye inowira pacrystal network. Nekuda kwerutsigiro rwemuchina, kudonha kunogona kudzivirirwa kunyangwe kuomesa tembiricha yakakwira kupfuura Tg' (kana kunyangwe Tc). Mukugadzirisa neprotein concentrations