Tlhahiso ea indasteri ea erythritol haholo-holo e sebelisa mokhoa oa ho belisoa ha likokoana-hloko, ho e-na le ho nt?a ka ho toba limela tsa tlhaho. Lebaka ke hobane erythritol e na le lintho tse tlase ka tlhaho, 'me ho nt?oa ka kotloloho ho bitsa chelete e ngata ebile ha ho sebetse hantle. Tse latelang ke lits'ebetso tsa mantlha le lintlha tsa tekheniki bakeng sa tlhahiso ea indasteri ea erythritol:

Mokhoa o ka sehloohong oa tlhahiso: Mokhoa oa ho belisoa ha likokoana-hloko
Khetho ea mefuta ea baktheria

Core strains: Erythritol e hlahisang haholo (jwalo ka Moniliella pollinis, Yarrowia lipolytica) kapa hlobo (jwalo ka Trichosporonoides megachiliensis).
Ntlafatso ea boenjiniere ba liphatsa tsa lefutso: Theknoloji ea morao-rao e ntlafatsa sekhahla sa phetoho ea joala ea tsoekere le khatello e phahameng ea osmotic ea mefuta ea baktheria ka phetoho ea liphatsa tsa lefutso.
Ts'ebetso ea thepa e tala

Mohloli oa carbon: Sebelisa motsoako oa tsoekere kapa sucrose (e nang le motsoako o fihlang ho 30-35%, ho theha tikoloho e phahameng ea osmotic ho nt?etsa pele motsoako oa erythritol).
Kalafo ea pele: Lisebelisoa tse theko e tlaase tse kang starch ea poone li hloka ho fetoloa tsoekere ka enzymatic hydrolysis (α - amylase + amylase).
Mokhoa oa ho belisa

Ho belisoa ho laoloang ke oksijene: Etsa hore ho belisoa ho tebileng ha moea ka har'a litanka tse kholo tsa ho belisoa, ho laola pH (5.0-6.0), mocheso (28-34 ℃), le boemo ba oksijene e qhibilihisitsoeng.
Ho kenya khatello e phahameng ea osmotic: Tlas'a khatello e phahameng ea tsoekere, likokoana-hloko li fetola tsoekere e 'ngoe ho erythritol ho leka-lekanya khatello ea sele ea osmotic.
Mehato ea bohlokoa bakeng sa karohano le tlhoekiso

Lintlha tsa tekheniki tsa mehato
Sterilization filtration, microfiltration kapa centrifugation ho tlosa lisele tsa baktheria le lit?ila tse kholo tsa limolek'hule.
Decolorization desalination activated carbon adsorbs pigments, ion exchange resin e tlosa letsoai le inorganic
Concentrated crystallization vacuum evaporation concentration to supersaturation → cooling crystallization (ho laola keketso ea peo le sekhahla sa ho pholisa)
Ho omisa ka centrifugal, karohano ea likristale tse bohareng → ho omisa bethe ho fumana likristale tse t?oeu ka bohloeki bo fetang 99.5%
Boteng ba tlhaho le ho nt?oa ho fokolang (e seng indasteri)
Leha tlhahiso ea indasteri e it?etleha ka ho belisoa, ka tlhaho erythritol e teng ho:

Litholoana: morara, lipere, mahapu (litaba tse ka bang 0.005-0.1%)
Lijo tse halikiloeng: soy sauce, sake
Mokhoa oa ho etsa liteko (bakeng sa lipatlisiso tsa mahlale feela):
mokgoahlapi
Kopitsa Khoutu
set?oant?o sa LR
A [Mohlala oa Ho Sila] -->B [Mokhoa oa Metsi a Chesang]
B -->C [Ethanol precipitation polysaccharides]
C -> D [ho hloekisa chromatography ea ion exchange]
D -> E [HPLC karohano ea erythritol]
Mathata: Kotulo e tlase haholo (Melemo e bapisoang le tlhahiso e meng ea joala ea tsoekere
Erythritol xylitol/sorbitol e ikhethang
Substrate ea fermentation glucose/sucrose hemicellulose hydrolyzate (xylose)
Bothata ba Crystallization: Ho bonolo ho kristale (solubility ea 37g/100g ho 25 ℃). Sorbitol e hloka ho benya butle butle ho thibela ho putlama
Mokhoa oa metabolic: 'mele oa motho ha o theohe (ka tlase ho 10% o monyelehile) xylitol e kenella ka mokhoa o itseng (insulin e nka karolo).
Khatelo-pele ea theknoloji
T?ebeliso ea lit?ila: Boiteko ba ho fetola tsoekere e hloekisitsoeng ka lignocellulose hydrolyzate ho fokotsa lit?enyehelo tsa thepa e tala ka ho feta 30%.
Ho belisoa ho tsoelang pele: Lera le lecha la bioreactor le fihlella tlhahiso e tsoelang pele, le khutsufatsa potoloho ea ho belisoa ho lihora tse 48.
Green crystallization: Theknoloji ea Ultrasonic e thusitseng crystallization e ntlafatsa bohloeki ba kristale (> 99.9%) mme e fokotsa t?ebeliso ea matla.
Qetello
"Ho nt?oa" ha erythritol ha e le hantle ho bolela mokhoa oa karohano ea ho belisoa ha indasteri, 'me ho nt?oa ha tlhaho ho na le moelelo oa mohopolo feela. Theknoloji ea morao-rao e sebelisa mefuta e entsoeng ka liphatsa tsa lefutso, ho belisoa ho hoholo, le mekhoa e mengata ea ho hloekisa ho finyella tlhahiso e theko e tlaase ea lithane tse limilione ka selemo, e finyellang tlhokahalo ea lijo tsa tsoekere. Haeba ho hlokahala lintlha tse itseng tsa t?ebetso kapa tlhahisoleseding ea patent e hlokahalang, t?ehetso e eketsehileng ea lingoliloeng e ka fanoa.