Ukuveliswa kwe-erythritol kwimizi-mveliso kusetyenziswa indlela yokuvundiswa kwe-microbial, kunokuba ikhutshwe ngokuthe ngqo kwizityalo zendalo. Oku kungenxa yokuba i-erythritol inomxholo ophantsi kwindalo, kwaye ukukhutshwa ngokuthe ngqo kuyabiza kwaye akusebenzi. Ezi zilandelayo ziinkqubo eziphambili kunye namanqaku obugcisa bokuvelisa i-erythritol kwimizi-mveliso:

Indlela yokuvelisa eyona nto iphambili: Indlela yokuvutshelwa kweMicrobial
Ukukhethwa kweentlobo zebhaktheriya

Iintlobo ezingundoqo: Igwele le-erythritol elivelisa kakhulu (elifana ne-Moniliella pollinis, i-Yarrowia lipolytica) okanye i-mold (efana ne-Trichosporonoides megachiliensis).
Ukulungiswa kobunjineli bemfuza: Itekhnoloji yale mihla yonyusa izinga lokuguqulwa kotywala beswekile kunye noxinzelelo oluphezulu lwe-osmotic yokumelana noxinzelelo lwebhaktheriya ngokuguqulwa kofuzo.
Ukusetyenzwa kwemathiriyeli ekrwada

Umthombo weCarbon: Sebenzisa i-glucose okanye isisombululo se-sucrose (kunye noxinzelelo ukuya kwi-30-35%, ukudala indawo ephezulu ye-osmotic ukukhuthaza i-erythritol synthesis).
Unyango lwangaphambili: Izinto ezingabizi kakhulu ezifana nesitashi sombona kufuneka ziguqulwe zibe yi-glucose nge-enzymatic hydrolysis (α - amylase + amylase).
Inkqubo yokuvundisa

Ukuvubela okulawulwa yioksijini: Ukuqhuba ukuvundisa okunzulu kwiitanki ezinkulu zokubilisa, lawula ngokungqongqo i-pH (5.0-6.0), ubushushu (28-34 ℃), kunye nenqanaba leoksijini enyibilikileyo.
Uxinzelelo oluphezulu lwe-osmotic induction: Ngaphantsi koxinzelelo lwe-glucose ephezulu, ii-microorganisms ziguqula i-glucose ibe yi-erythritol ukulinganisela uxinzelelo lwe-cell osmotic.
Amanyathelo aphambili okwahlula kunye nokuhlanjululwa

Iinkcukacha zobugcisa zamanyathelo
Ukuhluzwa kwe-sterilization, i-microfiltration okanye i-centrifugation ukususa iiseli zebhaktheriya kunye nokungcola okukhulu kweemolekyuli.
I-decolorization desalination isebenze i-carbon adsorbs pigments, i-ion exchange resin isusa iityuwa ezingaphili.
Ukugxilwa kwecrystallization vacuum evaporation yoxinaniso ukuya kwi-supersaturation → ukupholisa icrystallization (ukulawula ukongezwa kwembewu kunye nesantya sokupholisa)
Ukomiswa kwe-Centrifugal, ukwahlula phakathi kweekristale kwi-centrifugal → ukomiswa kwebhedi okumanzi ukufumana iikristale ezimhlophe ngobunyulu obungaphezulu kwe-99.5%
Ubukho bendalo kunye nokutsalwa kwesikali esincinci (akukho mveliso)
Nangona imveliso yemizi-mveliso ixhomekeke ekuvubeleni, i-erythritol ikhona ngokwendalo:

Iziqhamo: iidiliya, amapere, ivatala (umxholo malunga ne-0.005-0.1%)
Ukutya okubilisiweyo: i-soy sauce, i-sake
Indlela yokulinga yokutsalwa (ngeenjongo zophando lwenzululwazi kuphela):
unomakazi
IKhowudi yokukopa
igrafu LR
A [Isampula yokuSila] -->B [Ukutsalwa kwamanzi ashushu]
B -->C [Ethanol precipitation polysaccharides]
C -->D [ukuhlanjululwa kwechromatography yokutshintshiselana ngeion]
D -->E [Ukwahlulwa kwe-HPLC ye-erythritol]
Ukungalungi: Isivuno esiphantsi kakhulu (Iinzuzo zokuthelekisa nezinye imveliso yotywala iswekile
Uphawu lwe-erythritol xylitol/sorbitol
I-Fermentation substrate glucose/sucrose hemicellulose hydrolyzate (xylose)
Ubunzima beCrystallization: Kulula ukuqaqambisa (i-solubility ye-37g / 100g kwi-25 ℃). I-Sorbitol kufuneka icwebezelise kancinci ukunqanda ukuqumba
Indlela ye-Metabolic: Umzimba womntu awusebenzisi i-metabolize (Inkqubela phambili yezobuchwepheshe
Ukusetyenziswa kwenkunkuma: Umzamo wokutshintsha i-glucose esulungekileyo kunye ne-lignocellulose hydrolyzate ukunciphisa iindleko zempahla eluhlaza ngaphezu kwe-30%.
Ukuvutshelwa okuqhubekayo: I-membrane entsha ye-bioreactor ifezekisa imveliso eqhubekayo, inciphisa umjikelo wokuvutshelwa kwiiyure ezingama-48.
I-crystallization eluhlaza: I-ultrasonic incedise iteknoloji ye-crystallization iphucula ukucoceka kwe-crystal (> 99.9%) kunye nokunciphisa ukusetyenziswa kwamandla.
Ukuqukumbela
"Ukutsalwa" kwe-erythritol eneneni kubhekisa kwinkqubo yokwahlula i-industrial fermentation, kwaye ukutsalwa kwendalo kunokubaluleka kwethiyori kuphela. Itekhnoloji yale mihla isebenzisa iintlobo zobunjineli bemfuza, ukuvutshelwa okuphezulu, kunye neendlela ezininzi zokusulungekisa ukuphumeza imveliso enexabiso eliphantsi lezigidi zeetoni ngonyaka, ukuhlangabezana nemfuno yokutya kweswekile enguziro. Ukuba iiparameters zenkqubo ethile okanye ulwazi lwepatent luyafuneka, inkxaso eyongezelelekileyo yoncwadi inokubonelelwa.