Uphononongo lwangaphambili lubonise ukuba i-vitamin C ineenzuzo ezahlukeneyo zempilo, kubandakanywa amajoni omzimba, iziphumo ze-antioxidant, kunye nempilo ye-cardiovascular. Nangona i-vitamin C iyisondlo esibalulekileyo sokugcina impilo, kakhulu okanye encinci kunokuba nemiphumo emibi kwimpilo.

I-Melanoma (MM) lithumba eliyingozi elisuka kwiiseli ezinemibala yolusu kwaye lolona hlobo luyingozi kakhulu lomhlaza wolusu, nangona luneziganeko eziphantsi kwaye lunobundlobongela kunye ne-metastatic. Iziganeko ze-melanoma ziye zanda kule minyaka imbalwa idlulileyo.

Kutshanje, Abaphandi abavela kwiYunivesithi yaseMzantsi Florida eUnited States kunye neYunivesithi yaseLeicester eUnited Kingdom bapapashe iphepha elinesihloko esithi "Ukuguqulwa kweRedox yomonakalo owenziwe nge-oxidatively-induced DNA yi-ascorbate iphucula" kwijenali yasimahla yebhayoloji yebhayoloji kunye neyeza zombini kwi-vitro kunye ne-ex-vivo yokwakheka komonakalo we-DNA kunye nokufa kweseli kwiiseli ze-melanoma ".

Uphononongo lubonise ukuba ukunyanga iiseli zomhlaza we-melanoma ngevithamin C kunokonyusa umonakalo we-DNA owenziwe nge-oxidant kwiiseli zomhlaza kunye nokukhuthaza ukufa kweseli yomhlaza, kwaye lo monakalo ulingana nesixa se-melanin kwiiseli. Kwaye kwiiseli zesikhumba eziqhelekileyo, zidlala indima yokukhusela.

Kolu phononongo, abaphandi baseka iqela lemigca yeeseli zeMM ezinemibala eyahlukeneyo, basebenzise i-hydrogen peroxide njengemodeli ye-oxidant, kwaye bahlalutya ivithamin C ukunyusa amathuba okubulala iseli ye-melanoma ngokwandisa umonakalo weDNA owenziwe nge-oxidation.

Abaphandi bavavanya amanqanaba e-vitamin C-induced DNA umonakalo kwimizila yeeseli ezintlanu kwaye bafumanisa ukuba xa kuthelekiswa neeseli zesikhumba eziqhelekileyo, i-keratinocytes (HaCaT), amanqanaba omonakalo we-DNA engapheliyo ayephezulu ngokubanzi kuzo zonke iiseli ze-MM, ukwenzela ukuba umonakalo onzima: iiseli ze-SK23 ezinebala eliphezulu, iiseli ze-SK28 ezine-pigmentation ephakathi kunye neeseli ze-A3g5MP, iiseli ze-A3g5MP, i-A3g5MP, iiseli ze-A3g5MP, iiseli ze-A3g5MP. ibenelona qondo liphantsi lomonakalo weDNA.

Ukongezelela, abaphandi bahlalutya uvakalelo lwemigca emihlanu yeeseli kumonakalo owenziwe yi-oxidant (i-hydrogen peroxide) kwaye yafumanisa ukuba uvakalelo lomonakalo kwi-hydrogen peroxide lwaluhambelana noku ngasentla.

Uhlalutyo olongezelelweyo lweentlobo ze-oxidative ze-intracellular lubonise ukuba iiseli ze-MM zibonise amanqanaba aphezulu e-endogenous e-intracellular oxidatives kuneeseli ze-HaCaT, kwaye ukulandelelana kwemigca yeeseli ezintlanu kwakuhambelana nomonakalo we-DNA, uvakalelo lomonakalo, kunye ne-cytopigmentation.

Emva koko, abaphandi baphatha iiseli kunye okanye ngaphandle kwe-vitamin C kwaye bahlalutya iziphumo ezinokuthi zilawule i-vitamin C kwi-oxidation-induced DNA umonakalo wokwakhiwa kunye nokubulawa kweeseli.

Iziphumo zibonise ukuba kuzo zonke iiseli ze-MM, inqanaba lomonakalo we-DNA engapheliyo eyenziwa yi-vitamin C yonyango yanda kakhulu, ngelixa iiseli ze-HaCaT zazingabalulekanga, kwaye umonakalo we-DNA owenziweyo wawuhambelana noku ngasentla.

Ukongezelela, inqanaba le-vitamin C-induced nucleobase umonakalo laliphezulu kakhulu kwiiseli ze-SK23 ze-pigmented (18.5%) kwaye zisezantsi kwiiseli ze-A375P ezingenambala (14.2%).

Ngokusekelwe kwinto yokuba i-vitamin C inokuphucula i-peroxide eyenziwe yi-endogenous DNA umonakalo kunye nomonakalo we-nucleobase kwiiseli ze-MM, abaphandi baphinde bahlalutya umphumo we-vitamin C kwi-DNA yokuqhawula i-strand ephindwe kabini kwaye bafumanisa ukuba kuzo zonke iiseli ze-MM, inqanaba le-DNA ikhefu eliphindwe kabini elibangelwa unyango lwe-vitamin C linyuke kakhulu, kodwa kungekhona kwiiseli ze-HaCaT. Ulandelelwano lwemigca yeseli emihlanu isahambelana noku kungasentla.

Okubalulekileyo, abaphandi bahlalutya ukuba i-vitamin C iphucule ukufa kwe-MM cell ye-peroxid, kwaye yafumanisa ukuba i-vitamin C iphucula ukubulawa kwe-peroxid-induced ukubulawa kwazo zonke iiseli ze-MM, ngelixa idlala indima yokukhusela kwiiseli ze-HaCaT, kwaye ulandelelwano lokubulala lwaluhambelana noku ngasentla.

Okokugqibela, uphononongo lukwafumanise ukuba ivithamin C inokonyusa ukusebenza kwechiza elikhoyo lemelanoma i-Elesclomol, iphucula kakhulu umonakalo weDNA yeeseli zomhlaza obangelwa yi-Elesclomol.

Abaphandi bathi ukusebenzisa i-vitamin C, enokunyusa umonakalo we-DNA kwiiseli zomhlaza kwaye ikhokelela ekufeni kweseli yomhlaza, inokuba yindlela esebenzayo yokunyanga i-melanoma, esafuna izifundo zeklinikhi kunye nezilingo zokuqinisekisa.

Ngenxa yokuba i-vitamin C ifundwe kakuhle kwaye iyaziwa ukuba inyamezeleke kakuhle, abaphandi bakholelwa ukuba oogqirha banokusebenzisa i-vitamin C njengesongezelelo sokuphucula unyango olukhoyo.

Ithatyathwe kunye, olu phononongo lwe-in vitro lubonisa ukubaivithamin Cinokwandisa umonakalo we-DNA obangelwa yi-oxidation, ikhuthaze ukufa kweeseli zomhlaza, ngelixa idlala indima yokukhusela kwiiseli eziqhelekileyo zolusu, kwaye iphucule ukusebenza kakuhle kwamachiza e-melanoma asele ekhona, afanele ukuphononongwa ngakumbi.