Ukukhiqizwa kwezimboni kwe-erythritol kusebenzisa indlela yokuvutshelwa kwe-microbial, kunokuba ikhishwe ngokuqondile ezitshalweni zemvelo. Lokhu kungenxa yokuthi i-erythritol inokuqukethwe okuphansi ngokwemvelo, futhi ukukhipha okuqondile kuyabiza futhi akusebenzi kahle. Lokhu okulandelayo yizinqubo ezibalulekile kanye namaphuzu obuchwepheshe okukhiqizwa kwezimboni kwe-erythritol:

Indlela yokukhiqiza eyinhloko: Indlela yokuvutshelwa kwe-Microbial
Ukukhethwa kwezinhlobo zebhaktheriya

Izinhlobo eziyinhloko: I-erythritol yeast ekhiqiza kakhulu (njenge-Moniliella pollinis, Yarrowia lipolytica) noma isikhunta (njenge-Trichosporonoides megachiliensis).
Ukuthuthukiswa kobunjiniyela bezakhi zofuzo: Ubuchwepheshe besimanje buthuthukisa izinga lokuguqulwa kotshwala obushukela kanye nokumelana nengcindezi ephezulu ye-osmotic yezinhlobo zebhaktheriya ngokuguqulwa kofuzo.
Ukucubungula impahla eluhlaza

Umthombo wekhabhoni: Sebenzisa i-glucose noma isixazululo se-sucrose (ngokuhlangana okufika ku-30-35%, udale indawo ephezulu ye-osmotic ukukhuthaza ukuhlanganiswa kwe-erythritol).
Ukwelashwa kwangaphambili: Izinto zokusetshenziswa ezishibhile ezifana nesitashi sombila zidinga ukuguqulwa zibe ushukela nge-enzymatic hydrolysis (α - amylase+amylase).
Inqubo yokuvutshelwa

Ukuvutshelwa okulawulwa komoyampilo: Yenza ukuvutshelwa kwe-aeration okujulile emathangeni amakhulu okuvutshelwa, lawula ngokuqinile i-pH (5.0-6.0), izinga lokushisa (28-34 ℃), kanye nezinga le-oxygen elincibilikile.
Ukufakwa kwengcindezi ephezulu ye-osmotic: Ngaphansi kokugxilwa kweglucose ephezulu, ama-microorganisms aguqula i-glucose ethile ibe i-erythritol ukuze ibhalansise i-cell osmotic pressure.
Izinyathelo ezibalulekile zokuhlukanisa nokuhlanzwa

Imininingwane yobuchwepheshe yezinyathelo
Ukuhlunga inzalo, i-microfiltration noma i-centrifugation ukususa amangqamuzana ebhaktheriya nokungcola okukhulu kwamangqamuzana.
Decolorization desalination activated carbon adsorbs pigments, ion exchange resin isusa usawoti wezinto ezingaphili
Ukugxila kwe-crystallization vacuum evaporation okugxilisiwe kuya ekushiseni okuphezulu → ukupholisa ikristalu (ukulawula ukungezwa kwembewu nezinga lokupholisa)
Ukomiswa kwe-centrifugal, ukuhlukaniswa kwamakristalu ku-centrifugal → ukomiswa kombhede oketshezi ukuze kutholwe amakristalu amhlophe anobumsulwa obungaphezu kuka-99.5%
Ubukhona bemvelo kanye nokukhishwa kwesikali esincane (okungezona izimboni)
Nakuba ukukhiqizwa kwezimboni kuncike ekuvubeleni, i-erythritol ngokwemvelo ikhona ku:

Izithelo: amagilebhisi, amapheya, ikhabe (okuqukethwe mayelana 0.005-0.1%)
Ukudla okuvutshiwe: i-soy sauce, i-sake
Indlela yokuhlola yokukhipha (ngokwezinjongo zocwaningo lwesayensi kuphela):
ugogo
Kopisha Ikhodi
igrafu LR
A [Isampula Yokugaya] -->B [Isizinda Samanzi Ashisayo]
B -->C [Ethanol precipitation polysaccharides]
C -->D [i-ion exchange chromatography purification]
D -->E [ukuhlukaniswa kwe-HPLC kwe-erythritol]
Ukungalungi: Isivuno esiphansi kakhulu (Izinzuzo zokuqhathanisa nezinye ukukhiqizwa kotshwala obushukela
Isici se-erythritol xylitol/sorbitol
I-Fermentation substrate glucose/sucrose hemicellulose hydrolyzate (xylose)
Ubunzima be-Crystallization: Kulula ukucwebezela (i-solubility engu-37g/100g ku-25 ℃). I-Sorbitol idinga ukucwebezela kancane ukuze igweme ukunqwabelana
Indlela ye-Metabolic: Umzimba womuntu awugawuli i-metabolism (ngaphansi kwe-10% imuncwe) i-xylitol igaywa kancane (i-insulin ibamba iqhaza)
Inqubekelaphambili yezobuchwepheshe
Ukusetshenziswa kukadoti: Umzamo wokushintsha i-glucose ecolisisiwe ufake i-lignocellulose hydrolyzate ukuze unciphise izindleko zempahla eluhlaza ngaphezu kwama-30%.
Ukuvutshelwa okuqhubekayo: I-membrane bioreactor entsha izuza ukukhiqizwa okuqhubekayo, ifinyeza umjikelezo wokuvutshelwa ube amahora angu-48.
I-crystallization eluhlaza: Ubuchwepheshe be-crystallization obusizwa nge-ultrasonic buthuthukisa ukuhlanzeka kwekristalu (> 99.9%) futhi kunciphisa ukusetshenziswa kwamandla.
Isiphetho
"Ukukhishwa" kwe-erythritol empeleni kubhekisela enqubweni yokuhlukanisa ukuvutshelwa kwemboni, futhi isizinda semvelo sinokubaluleka okungokwethiyori kuphela. Ubuchwepheshe besimanje busebenzisa izinhlobo ezakhiwe ngofuzo, ukuvutshelwa okugxilile okukhulu, nezindlela eziningi zokucwenga ukuze kuzuzwe ukukhiqizwa okungabizi kakhulu kwezigidi zamathani ngonyaka, kuhlangatshezwane nesidingo sokudla okushukela okuyiziro. Uma amapharamitha enqubo ethile noma ulwazi lwelungelo lobunikazi ludingeka, ukusekelwa okwengeziwe kwezincwadi kunganikezwa.