Ucwaningo lwangaphambili lubonise ukuthi uvithamini C unezinzuzo ezihlukahlukene zezempilo, okuhlanganisa amasosha omzimba, imiphumela ye-antioxidant, nempilo yenhliziyo. Nakuba i-vitamin C iyisakhi esibalulekile sokugcina impilo, okuningi noma okuncane kakhulu kungaba nemiphumela emibi empilweni.

I-Melanoma (MM) iyithumba eliyingozi elivela kumangqamuzana esikhumba anombala futhi iwuhlobo oluyingozi kakhulu lomdlavuza wesikhumba, nakuba inezinga eliphansi futhi inolaka kakhulu futhi i-metastatic. Izibalo ze-melanoma ziye zanda emashumini ambalwa eminyaka adlule.

Muva nje, Abacwaningi abavela eNyuvesi yaseNingizimu Florida e-United States nase-University of Leicester e-United Kingdom bashicilele iphepha elinesihloko esithi "Redox modulation of oxidatively-induced DNA damage by ascorbate enhances" ephephabhukwini I-Free Radical Biology and Medicine kokubili ku-vitro kanye nokwakhiwa komonakalo we-DNA yangaphambili kanye nokufa kwamangqamuzana kumaseli e-melanoma ".

Ucwaningo luye lwabonisa ukuthi ukwelapha amangqamuzana omdlavuza we-melanoma ngovithamini C kungakhuphula ukulimala kwe-DNA ebangelwa i-oxidant kumangqamuzana omdlavuza futhi kukhuthaze ukufa kwamangqamuzana omdlavuza, futhi lo monakalo ulingana nenani le-melanin emangqamuzaneni. Futhi kumaseli esikhumba avamile, idlala indima yokuzivikela.

Kulolu cwaningo, abacwaningi basungula iqembu lemigqa yamaseli e-MM ane-pigmentation ehlukene, basebenzisa i-hydrogen peroxide njenge-oxidant eyimodeli, futhi bahlaziya i-vitamin C ukuze bakhulise amathuba okubulala ingqamuzana le-melanoma ngokuthuthukisa umonakalo we-DNA owenziwe nge-oxidation.

Abacwaningi bahlole amazinga okulimala kwe-DNA eyenziwe nge-vitamin C emigqeni emihlanu yamaseli futhi bathola ukuthi uma kuqhathaniswa namangqamuzana esikhumba avamile, ama-keratinocytes (HaCaT), amazinga okulimala kwe-DNA endogenous ngokuvamile ayephakeme kuwo wonke amangqamuzana e-MM, ukuze kube nobukhulu bomonakalo: amaseli e-SK23 ane-pigmentation ephezulu, amaseli e-SK28 ane-pigmentation ephakathi, amangqamuzana e-A37M angenayo i-pigmentation ye-A375, i-A375MP, i-A375. yayinezinga eliphansi kakhulu lokulimala kwe-DNA.

Ngaphezu kwalokho, abacwaningi bahlaziye ukuzwela kwemigqa yeseli emihlanu ekulimazeni okubangelwa i-oxidant (i-hydrogen peroxide) futhi bathola ukuthi ukuzwela komonakalo ku-hydrogen peroxide kwakuhambisana nalokhu okungenhla.

Ukuhlaziywa okwengeziwe kwezinhlobo ze-intracellular oxidative kubonise ukuthi amaseli e-MM abonise amazinga aphezulu kakhulu e-endogenous ezinhlobo ze-oxidative ze-intracellular kunamaseli e-HaCaT, futhi ukulandelana kwemigqa yamaseli amahlanu kwakuhambisana nokulimala kwe-DNA, ukuzwela kokulimala, kanye ne-cytopigmentation.

Okulandelayo, abacwaningi baphathe amaseli nge-vitamin C noma ngaphandle kwayo futhi bahlaziya imiphumela engaba khona yokulawula kavithamini C ekwakhekeni komonakalo we-DNA owenziwe nge-oxidation kanye nokubulawa kwamaseli.

Imiphumela yabonisa ukuthi kuwo wonke amangqamuzana e-MM, izinga lokulimala kwe-DNA endogenous elenziwe ukwelashwa kwe-vitamin C lakhuphuka kakhulu, kuyilapho amangqamuzana e-HaCaT ayengabalulekile, futhi umonakalo owenziwe we-DNA ongapheli wawuhambisana nalokhu okungenhla.

Ngaphezu kwalokho, izinga lomonakalo we-nucleobase owenziwe nge-vitamin C laliphezulu kakhulu kumaseli e-SK23 anemibala eminingi (18.5%) futhi aphansi kakhulu kumaseli we-A375P angenayo i-pigmented (14.2%).

Ngokusekelwe eqinisweni lokuthi i-vitamin C ingathuthukisa ukulimala kwe-DNA ye-peroxide eyenziwe endogenous kanye nokulimala kwe-nucleobase kumaseli e-MM, abacwaningi baphinde bahlaziya umphumela we-vitamin C kuma-DNA ama-strand ama-breaks aphindwe kabili futhi bathola ukuthi kuwo wonke amaseli e-MM, izinga le-DNA yokuhlukana kwemicu ephindwe kabili ebangelwa ukwelashwa kwe-vitamin C lenyuke kakhulu, kodwa hhayi kumaseli e-HaCaT. Ukulandelana kwemigqa yamaseli amahlanu kusahambisana nalokhu okungenhla.

Okubalulekile, abacwaningi bahlaziya ukuthi i-vitamin C ithuthukise ukufa kwe-MM cell eyenziwe nge-peroxid, futhi bathola ukuthi i-vitamin C ithuthukise ukubulawa kwe-peroxid-induced yawo wonke amaseli e-MM, ngenkathi idlala indima yokuvikela kumaseli e-HaCaT, futhi ukulandelana kokubulala kwakuhambisana nalokhu okungenhla.

Ekugcineni, ucwaningo luphinde lwathola ukuthi i-vitamin C ingathuthukisa ukusebenza kwesidakamizwa esikhona se-melanoma i-Elesclomol, ikhulise kakhulu umonakalo we-DNA wamangqamuzana omdlavuza obangelwa i-Elesclomol.

Abacwaningi bathi ukusebenzisa uvithamini C, ongandisa ukulimala kwe-DNA kumangqamuzana omdlavuza futhi kuholele ekufeni kwamangqamuzana omdlavuza, kungase kube indlela ephumelela kakhudlwana yokwelapha i-melanoma, esadinga izifundo eziningi zemitholampilo kanye nokuhlolwa ukuze kuqinisekiswe.

Njengoba kunikezwe ukuthi uvithamini C ufundwe kahle futhi waziwa ukuthi ubekezelelwa kahle, abacwaningi bakholelwa ukuthi odokotela bangasebenzisa uvithamini C njengesithasiselo sokuthuthukisa izindlela zokwelapha ezikhona.

Sekuhlangene, lolu cwaningo lwe-in vitro lukhombisa lokhouvithamini Cingathuthukisa ukulimala kwe-DNA ye-endogenous eyenziwe nge-oxidation, ikhuthaze ukufa kwamangqamuzana omdlavuza, kuyilapho idlala indima yokuvikela kumangqamuzana esikhumba avamile, futhi ithuthukise ukusebenza kahle kwezidakamizwa ezikhona ze-melanoma, okufanele kuqhutshekwe nocwaningo.